Nitrotetrazolium Blue Chloride CAS: 298-83-9

Nitrotetrazolium Blue Chloride (NBT) yog ib qho kev qhia redox feem ntau siv hauv kev tshuaj lom neeg thiab tshuaj lom neeg.Nws yog cov hmoov daj daj uas tig xiav thaum txo qis, ua rau nws muaj txiaj ntsig zoo rau kev kuaj pom muaj qee yam enzymes thiab kev ua haujlwm metabolic.

Thawj cov nyhuv ntawm NBT yog tsim ntawm xiav formazan precipitate thaum nws raug txo los ntawm qee cov enzymes.Qhov kev hloov xim no tso cai rau kev pom lossis spectrophotometric nrhiav pom ntawm enzyme kev ua haujlwm.

NBT muaj ntau yam kev siv hauv kev tshawb fawb thiab kev kuaj mob.Nov yog qee qhov ntawm nws cov kev siv tseem ceeb:

Enzyme kev ntsuam xyuas: NBT tuaj yeem siv los ntsuas kev ua haujlwm ntawm dehydrogenases, uas koom nrog cov txheej txheem xws li kev ua pa ntawm tes thiab cov metabolism.Los ntawm kev saib xyuas qhov txo qis ntawm NBT mus rau formazan, cov kws tshawb fawb tuaj yeem soj ntsuam cov haujlwm ntawm cov enzymes no.

Kev soj ntsuam ntawm lub cev tiv thaiv kab mob ua haujlwm: NBT feem ntau yog siv hauv NBT txo qhov ntsuas los ntsuas qhov ua pa tawg ntawm lub cev tiv thaiv kab mob, tshwj xeeb tshaj yog phagocytes.Qhov kev sim ntsuas lub peev xwm ntawm cov hlwb no los tsim cov pa oxygen reactive, uas tuaj yeem txo NBT thiab tsim cov xim xiav.

Kev tshawb fawb microbiology: NBT yog ua haujlwm hauv microbiology los kawm microbial metabolism thiab tshuaj xyuas cov haujlwm ntawm cov enzymes tshwj xeeb.Piv txwv li, nws tau raug siv los kuaj cov kab mob nitrate reductases lossis cov kab mob formazan-forming.

Kev tshawb fawb txog cell viability: NBT txo qis tso cai rau cov kws tshawb fawb los soj ntsuam cov haujlwm metabolic thiab kev muaj peev xwm ntawm cov hlwb.Los ntawm kev txheeb xyuas qhov kev siv ntawm cov khoom xiav formazan, nws muaj peev xwm txiav txim siab tus naj npawb ntawm cov hlwb siv tau hauv cov qauv muab.