4-Methylumbelliferyl-beta-D-glucopyranoside CAS: 18997-57-4

Cov nyhuv ntawm 4-Methylumbelliferyl-beta-D-glucopyranoside (MUG) yog ua haujlwm ua lub substrate rau cov enzyme beta-glucosidase. Cov enzyme no txiav cov glucosidic bond ntawm MUG, ua rau muaj kev tso tawm ntawm 4-Methylumbelliferone (4-MU). Daim ntawv thov ntawm MUG yog feem ntau hauv thaj chaw ntawm microbiology, tshwj xeeb tshaj yog rau kev kuaj pom thiab txheeb xyuas cov kab mob beta-glucosidase. MUG feem ntau siv rau hauv kev kuaj pom Escherichia coli (E. coli) hauv cov dej thiab cov khoom noj. E. coli muaj cov enzyme beta-glucosidase, uas tuaj yeem hydrolyze MUG thiab tsim cov teeb liab fluorescent nyob rau hauv qhov muaj ultraviolet (UV) lub teeb. Cov khoom fluorescent ntawm 4-MU tso cai rau kev kuaj pom yooj yim thiab kev ntsuas. Thaum lub substrate MUG yog hydrolyzed, 4-MU tsim tawm emits xiav fluorescence, pab txhawb kev txheeb xyuas cov kab mob uas muaj beta-glucosidase kev ua ub no. Txoj kev no feem ntau siv rau hauv kev saib xyuas ib puag ncig thiab kev kuaj xyuas kev nyab xeeb zaub mov, vim nws muab txoj hauv kev ceev thiab rhiab heev los ntes cov kab mob sib kis. Ntxiv rau nws daim ntawv thov hauv microbiology, MUG kuj tseem siv tau los kawm txog kev ua haujlwm thiab kev txwv ntawm beta-glucosidase hauv biochemistry thiab molecular biology kev tshawb fawb. Nws cov fluorescence ua rau kev ntsuas ntawm enzyme kinetics thiab tuaj yeem siv los tshuaj xyuas cov tshuaj inhibitors lossis activators ntawm beta-glucosidase kev ua haujlwm. Zuag qhia tag nrho, MUG yog ib qho tshuaj sib xyaw ua ke uas pom cov ntawv thov dav dav hauv cov teb ntawm microbiology, biochemistry, thiab molecular biology rau kev ntes ntawm beta-glucosidase kev ua haujlwm thiab kev txheeb xyuas cov kab mob uas tsim cov enzyme no.