4-Nitrophenyl beta-D-galactopyranoside CAS: 200422-18-0

Cov Nyhuv: ONPG yog ib qho substrate tshwj xeeb siv los ntes qhov muaj thiab kev ua haujlwm ntawm cov enzyme β-galactosidase. Thaum β-galactosidase enzyme muaj thiab ua haujlwm, nws txiav ONPG ua ob yam khoom: o-nitrophenol thiab galactose derivative. Kev tso tawm ntawm o-nitrophenol ua rau muaj kev hloov xim daj, uas tuaj yeem ntsuas tau siv lub spectrophotometer.

Daim Ntawv Thov: ONPG muaj ntau daim ntawv thov hauv kev tshawb fawb txog molecular biology thiab biochemistry:

Kev txiav txim siab ntawm β-galactosidase kev ua haujlwm: ONPG feem ntau yog siv los ntsuas thiab ntsuas qhov kev ua haujlwm ntawm β-galactosidase enzyme. Tus nqi ntawm o-nitrophenol tsim, uas yog ncaj qha proportional rau qhov kev ua haujlwm ntawm enzyme, tuaj yeem ntsuas spectrophotometrically.

Kev qhia tawm thiab kev tswj hwm cov noob caj noob ces: ONPG feem ntau siv rau hauv kev sim cuam tshuam nrog kev qhia tawm thiab kev tswj hwm cov noob caj noob ces. Nws feem ntau siv rau hauv kev sim fusion protein, xws li lub lacZ fusion system uas siv ntau, los kawm txog kev qhia tawm ntawm cov noob caj noob ces nyob rau hauv kev tswj hwm ntawm cov neeg txhawb nqa tshwj xeeb. Cov haujlwm beta-galactosidase ntsuas siv ONPG muab kev nkag siab rau theem ntawm kev qhia tawm ntawm cov noob caj noob ces.

Kev tshuaj xyuas rau β-galactosidase kev ua haujlwm: ONPG tuaj yeem siv ua txoj kev tshuaj xyuas xim hauv cov thev naus laus zis DNA recombinant los txheeb xyuas qhov muaj thiab ua haujlwm ntawm LacZ gene, uas encodes β-galactosidase. Txoj kev tshuaj xyuas no pab txheeb xyuas cov clones uas muaj cov gene ntawm kev txaus siab.

Kev tshawb fawb txog enzyme kinetics: ONPG kuj tseem muaj txiaj ntsig zoo rau kev kawm txog kinetics ntawm β-galactosidase enzyme. Los ntawm kev ntsuas qhov nrawm ntawm enzyme-substrate reaction ntawm ntau qhov sib txawv substrate concentration, nws muaj peev xwm txiav txim siab kinetic parameters xws li Michaelis-Menten constants (Km) thiab qhov siab tshaj plaws reaction rates (Vmax).