ONPG CAS: 369-07-3 Tus nqi tsim khoom

Qhov cuam tshuam ntawm ONPG ua ib qho substrate yog yuav tsum tau cleaved los ntawm cov enzyme β-galactosidase, ua rau muaj kev tso tawm ntawm cov khoom daj, o-nitrophenol. Qhov kev hloov xim no tuaj yeem ntsuas spectrophotometrically, tso cai rau kev ntsuas ntawm β-galactosidase kev ua ub no. Daim ntawv thov ntawm ONPG yog qhov tseem ceeb hauv kev ntsuam xyuas kev qhia txog gene hauv kev tshawb fawb molecular biology thiab microbiology. Nws feem ntau yog siv los ntsuas β-galactosidase kev ua ub no ua tus neeg sau xov xwm rau kev tshawb fawb txog kev qhia txog gene, tshwj xeeb tshaj yog hauv cov kab mob xws li E. coli. Lub lacZ gene, uas encodes β-galactosidase, feem ntau yog siv ua tus cim rau kev tshuaj xyuas kev qhia txog gene, vim nws qhov kev qhia tuaj yeem raug tsim los ntawm cov xwm txheej tshwj xeeb lossis tswj los ntawm cov neeg txhawb nqa tshwj xeeb. Qhov kev sim ONPG muab ib txoj hauv kev yooj yim thiab txhim khu kev qha los ntsuas qib ntawm kev qhia txog gene los ntawm kev ntsuas qhov kev ua ub no ntawm β-galactosidase. Qhov kev sim no yog siv dav hauv ntau yam kev siv, xws li kev kawm txog kev ua ub no ntawm cov neeg txhawb nqa, kev tswj hwm gene, thiab kev sib cuam tshuam ntawm cov protein-protein. Tsis tas li ntawd xwb, nws siv tau los txiav txim siab txog enzyme kinetics thiab soj ntsuam cov teebmeem ntawm kev hloov pauv lossis kev kho mob rau kev ua haujlwm ntawm enzyme.