Nitrotetrazolium Xiav Chloride CAS: 298-83-9

Nitortrazolium Blue Chloride (NBT) yog ib qho redox indicator uas feem ntau siv rau hauv kev ntsuam xyuas biological thiab biochemical. Nws yog ib qho hmoov daj daj uas hloov xiav thaum txo qis, ua rau nws muaj txiaj ntsig zoo rau kev kuaj pom qee yam enzymes thiab kev ua haujlwm metabolic.

Qhov cuam tshuam tseem ceeb ntawm NBT yog kev tsim cov xim xiav formazan thaum nws raug txo los ntawm qee cov enzymes. Qhov kev hloov xim no tso cai rau kev pom lossis spectrophotometric pom ntawm kev ua haujlwm ntawm enzyme.

NBT muaj ntau yam kev siv hauv kev tshawb fawb thiab kev kuaj mob. Nov yog qee qhov kev siv tseem ceeb:

Kev ntsuam xyuas kev ua haujlwm ntawm cov enzyme: NBT siv tau los ntsuas kev ua haujlwm ntawm dehydrogenases, uas koom nrog cov txheej txheem xws li kev ua pa ntawm tes thiab kev ua haujlwm ntawm lub cev. Los ntawm kev saib xyuas kev txo qis ntawm NBT rau formazan, cov kws tshawb fawb tuaj yeem ntsuas kev ua haujlwm ntawm cov enzymes no.

Kev ntsuam xyuas kev ua haujlwm ntawm cov hlwb tiv thaiv kab mob: NBT feem ntau siv rau hauv kev sim txo NBT los ntsuam xyuas kev ua pa ntawm cov hlwb tiv thaiv kab mob, tshwj xeeb tshaj yog phagocytes. Qhov kev sim ntsuas qhov peev xwm ntawm cov hlwb no los tsim cov tshuaj reactive oxygen, uas tuaj yeem txo NBT thiab tsim cov dej xiav.

Kev tshawb fawb txog kab mob me me: NBT siv rau hauv kev tshawb fawb txog kab mob me me los kawm txog kev ua haujlwm ntawm cov kab mob me me thiab soj ntsuam cov haujlwm ntawm cov enzymes tshwj xeeb. Piv txwv li, nws tau siv los nrhiav cov kab mob nitrate reductases lossis cov kab mob formazan-forming.

Kev tshawb fawb txog kev muaj sia nyob ntawm cov cell: Kev txo qis NBT tso cai rau cov kws tshawb fawb los ntsuam xyuas cov haujlwm metabolic thiab kev muaj sia nyob ntawm cov cell. Los ntawm kev ntsuas qhov muaj zog ntawm cov khoom xiav formazan, nws muaj peev xwm txiav txim siab tus naj npawb ntawm cov cell muaj sia nyob hauv ib qho qauv.