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CAS: 108321-07-9
Piperazine-N,N'-bis(2-hydroxypropanesulphonic acid) disodium ntsev yog ib qho tshuaj sib xyaw ua ke ntawm piperazine, bis(2-hydroxypropanesulphonic acid) pawg, thiab ob lub sodium ions. Nws feem ntau siv ua tus neeg sawv cev buffering thiab pH regulator hauv ntau yam kev siv hauv kev lag luam thiab chaw kuaj mob. Cov tshuaj no pab tswj kom muaj pH tshwj xeeb hauv cov tshuaj, ua rau nws muaj txiaj ntsig zoo hauv cov txheej txheem xws li kev ntxuav cov protein, molecular biology, thiab kev tshawb fawb txog tshuaj. Tsis tas li ntawd, nws kuj tseem tuaj yeem ua tus neeg sawv cev chelating rau cov hlau ions thiab ua kom cov enzyme ua haujlwm ruaj khov hauv qee yam kev tshuaj lom neeg.
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3-Morpholino-2-hydroxypropanesulfonic acid sodium ntsev CAS: 79803-73-9
3-Morpholino-2-hydroxypropanesulfonic acid sodium ntsev, tseem hu ua MES sodium ntsev, yog ib qho tshuaj lom neeg feem ntau siv ua tus neeg sawv cev buffering hauv kev tshawb fawb txog tsiaj txhu thiab biochemical.
MES yog ib qho zwitterionic buffer uas ua haujlwm ua tus tswj pH, ua kom pH ruaj khov hauv ntau lub tshuab sim. Nws yaj tau zoo heev hauv dej thiab muaj tus nqi pKa ntawm kwv yees li 6.15, ua rau nws tsim nyog rau buffering hauv pH ntawm 5.5 txog 7.1.
MES sodium ntsev feem ntau siv rau hauv cov txheej txheem molecular biology xws li DNA thiab RNA cais, enzyme assays, thiab protein purification. Nws kuj tseem siv rau hauv cell culture media kom tswj tau qhov pH ib puag ncig ruaj khov rau kev loj hlob thiab kev nthuav dav ntawm cell.
Ib qho tseem ceeb ntawm MES yog nws qhov ruaj khov nyob rau hauv cov xwm txheej ntawm lub cev thiab tiv taus kev hloov pauv ntawm qhov kub thiab txias. Qhov no ua rau nws tsim nyog rau kev siv hauv kev sim uas xav tias yuav muaj kev hloov pauv ntawm qhov kub thiab txias.
Cov kws tshawb nrhiav feem ntau nyiam MES sodium ntsev ua ib qho buffer vim nws qhov kev cuam tshuam tsawg kawg nkaus nrog cov tshuaj tiv thaiv enzymatic thiab muaj peev xwm buffer siab hauv nws qhov pH zoo tshaj plaws.
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X-GAL CAS: 7240-90-6 Tus nqi tsim khoom
5-Bromo-4-chloro-3-indolyl-β-D-galactoside (X-Gal) yog ib qho chromogenic substrate uas siv rau hauv molecular biology thiab biochemistry applications. Nws yog siv dav rau kev nrhiav pom ntawm lacZ gene, uas encodes lub enzyme β-galactosidase.
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1,2,3,4,6-Penta-O-acetyl-D-mannopyranose CAS: 25941-03-1
1,2,3,4,6-Penta-O-acetyl-D-mannopyranose yog ib qho tshuaj sib xyaw ua los ntawm D-mannose, ib qho suab thaj yooj yim. Nws yog ib qho derivative uas acetyl pawg txuas nrog tsib ntawm rau pawg hydroxyl uas muaj nyob rau hauv lub molecule mannose. Daim ntawv acetylated ntawm D-mannose no feem ntau siv rau hauv kev tsim cov organic thiab kev tshawb fawb txog tshuaj lom neeg ua lub tsev thaiv lossis cov khoom pib rau kev tsim cov molecules nyuaj dua. Cov pawg acetyl muab kev ruaj khov thiab tuaj yeem hloov pauv qhov reactivity thiab cov khoom ntawm cov tshuaj.
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1,2,3,4-Di-O-Isopropylidene-alpha-D-galactopyranose CAS: 4064-06-6
1,2:3,4-Di-O-isopropylidene-D-galactopyranose yog ib qho tshuaj lom neeg uas yog tsev neeg ntawm galactopyranose derivatives. Nws feem ntau siv hauv organic chemistry ua tus neeg sawv cev tiv thaiv rau cov hydroxyl pawg uas muaj nyob rau hauv cov suab thaj, tshwj xeeb tshaj yog galactose. Cov tshuaj no yog synthesized los ntawm reacting D-galactose nrog acetone los tsim ib qho diacetone derivative, uas tom qab ntawd kho nrog acid los tsim ib qho di-O-isopropylidene derivative. Cov derivative no tiv thaiv cov hydroxyl pawg, tiv thaiv cov tshuaj tsis xav tau thaum lub sijhawm tshuaj synthesis, thiab tuaj yeem raug tshem tawm xaiv los rov ua dua cov tshuaj qub. Nws cov qauv compact thiab kev ruaj khov ua rau nws muaj txiaj ntsig zoo hauv ntau yam kev siv hauv thaj chaw ntawm cov organic synthesis.
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PNPG CAS: 3150-24-1 Tus nqi tsim khoom
PNPG, lossis p-nitrophenyl β-D-glucopyranoside, yog ib qho molecule substrate me me uas feem ntau siv rau hauv biochemical assays los ntsuas qhov ua haujlwm ntawm glucosidase enzymes. Nws tsis muaj xim thiab tsis fluorescent, tab sis thaum hydrolysis los ntawm glucosidase, nws hloov mus ua p-nitrophenol, uas muaj xim daj thiab yooj yim pom spectrophotometrically.
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ONPG CAS: 369-07-3 Tus nqi tsim khoom
O-nitrophenyl-β-D-galactopyranoside (ONPG) yog ib qho khoom siv hluavtaws uas siv rau hauv kev tshuaj ntsuam biochemical thiab molecular biology los ntsuas qhov ua haujlwm ntawm cov enzyme β-galactosidase. Nws feem ntau siv rau hauv kev tshuaj ntsuam los ntes cov noob caj noob ces hauv cov kab mob bacterial, xws li Escherichia coli. ONPG yog ib qho tshuaj tsis muaj xim uas raug β-galactosidase txiav, ua rau muaj cov tshuaj daj, o-nitrophenol tso tawm. Cov xim daj uas tsim tawm tuaj yeem ntsuas spectrophotometrically, muab kev ntsuas tsis ncaj qha ntawm cov haujlwm enzyme. Kev tshuaj ntsuam siv ONPG feem ntau hu ua ONPG assay thiab yog ib txoj kev siv dav hauv kev tshawb fawb molecular biology los ntsuas cov qib kev qhia tawm ntawm cov noob caj noob ces uas tswj los ntawm lac operon hauv cov hlwb kab mob.
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Nitrotetrazolium Xiav Chloride CAS: 298-83-9
Nitortrazolium Blue Chloride (NBT) yog ib qho redox indicator uas feem ntau siv rau hauv kev ntsuam xyuas biological thiab biochemical. Nws yog ib qho hmoov daj daj uas hloov xiav thaum txo qis, ua rau nws muaj txiaj ntsig zoo rau kev kuaj pom qee yam enzymes thiab kev ua haujlwm metabolic.
NBT ua rau cov electron carriers thiab enzymes xws li dehydrogenases, uas koom nrog ntau yam txheej txheem cellular. Thaum NBT raug txo los ntawm cov enzymes no, nws tsim cov xiav formazan precipitate, uas tso cai rau kev pom lossis spectrophotometric nrhiav.
Cov tshuaj reagent no feem ntau siv rau hauv kev sim xws li kev sim txo NBT, qhov twg nws siv los ntsuas qhov ua haujlwm ntawm cov hlwb tiv thaiv kab mob. Nws kuj tseem siv tau los kawm txog cov haujlwm enzyme thiab cov txheej txheem metabolic hauv kev tshawb fawb txog kev ntxhov siab oxidative, kev muaj sia nyob ntawm lub hlwb, thiab kev sib txawv ntawm lub hlwb.
NBT tau pom cov ntawv thov hauv ntau qhov chaw, suav nrog microbiology, immunology, thiab cell biology. Nws muaj ntau yam siv, ruaj khov, thiab yooj yim siv, ua rau nws yog qhov kev xaiv nrov rau ntau cov txheej txheem sim.
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HATU CAS: 148893-10-1 Tus nqi tsim khoom
HATU (1-[bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxid hexafluorophosphate) yog ib qho tshuaj siv sib txuas ua ke hauv peptide synthesis thiab organic chemistry.
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D-fucose CAS: 3615-37-0 Tus nqi tsim khoom
D-fucose yog ib qho monosaccharide, tshwj xeeb yog ib qho suab thaj uas muaj rau lub carbon, uas yog ib feem ntawm pawg suab thaj yooj yim hu ua hexoses. Nws yog ib qho isomer ntawm glucose, sib txawv ntawm qhov kev teeb tsa ntawm ib pawg hydroxyl.
D-fucose muaj nyob rau hauv ntau yam kab mob, suav nrog cov kab mob bacteria, fungi, nroj tsuag, thiab tsiaj txhu. Nws ua lub luag haujlwm tseem ceeb hauv ntau yam txheej txheem ntawm lub cev, xws li kev xa xov ntawm lub hlwb, kev sib txuas ntawm lub hlwb, thiab kev tsim cov glycoprotein. Nws yog ib feem ntawm glycolipids, glycoproteins, thiab proteoglycans, uas koom nrog kev sib txuas lus thiab kev lees paub ntawm lub hlwb.
Hauv tib neeg, D-fucose kuj tseem koom nrog hauv biosynthesis ntawm cov qauv glycan tseem ceeb, xws li Lewis antigens thiab cov ntshav pawg antigens, uas muaj feem cuam tshuam rau kev sib raug zoo ntawm cov ntshav thiab kev kis kab mob.
D-fucose tuaj yeem tau los ntawm ntau qhov chaw, suav nrog seaweed, nroj tsuag, thiab microbial fermentation. Nws yog siv rau hauv kev tshawb fawb thiab kev siv biomedical, nrog rau kev tsim cov tshuaj thiab cov tshuaj kho mob.
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DDT CAS: 3483-12-3 Tus nqi tsim khoom
DL-Dithiothreitol, tseem hu ua DTT, yog ib qho tshuaj txo qis uas feem ntau siv rau hauv kev tshawb fawb txog biochemical thiab molecular biology. Nws yog ib qho molecule me me uas muaj ib pawg thiol (muaj sulfur) nyob rau txhua qhov kawg.
DTT feem ntau siv los rhuav tshem cov disulfide bonds hauv cov protein, uas pab kom lawv nthuav tawm lossis ua rau lawv tsis zoo. Qhov kev txo qis ntawm cov disulfide bonds no yog qhov tseem ceeb hauv ntau yam txheej txheem hauv chaw kuaj mob xws li kev ntxuav cov protein, gel electrophoresis, thiab kev kawm txog cov qauv protein. DTT kuj tseem siv tau los tiv thaiv cov pab pawg thiol thiab tiv thaiv kev oxidation thaum lub sijhawm sim.
Feem ntau DTT raug ntxiv rau hauv cov tshuaj sim hauv cov concentration me me, thiab nws cov haujlwm yog nyob ntawm qhov muaj oxygen. Nws yog ib qho tseem ceeb uas yuav tsum tau ua tib zoo saib xyuas DTT vim nws rhiab heev rau huab cua, cua sov, thiab dej noo, uas tuaj yeem txo nws cov txiaj ntsig.
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D-(+)-Galactose CAS:59-23-4 Tus nqi tsim khoom
D-(+)-Galactose yog ib hom suab thaj monosaccharide thiab yog ib qho tseem ceeb ntawm ntau yam txheej txheem ntawm lub cev. Nws yog ib hom suab thaj uas tshwm sim ntuj uas pom muaj nyob rau hauv ntau yam khoom noj, xws li txiv hmab txiv ntoo, khoom noj mis nyuj, thiab zaub.
Galactose feem ntau yog metabolized hauv lub cev los ntawm ntau yam kev hloov pauv enzymatic. Nws ua lub luag haujlwm tseem ceeb hauv kev sib txuas lus ntawm tes, kev tsim hluav taws xob, thiab kev tsim cov molecules tseem ceeb xws li glycolipids, glycoproteins, thiab lactose.
Hais txog nws daim ntawv thov, D-(+)-Galactose feem ntau siv rau hauv microbiology thiab biotechnology ua lub hauv paus carbon hauv cov kab lis kev cai rau kev loj hlob ntawm ntau yam kab mob me me. Nws kuj tseem siv rau hauv kev tsim ntau yam bioactive compounds, tshuaj, thiab cov khoom noj. Tsis tas li ntawd, nws feem ntau siv ua tus neeg sawv cev kuaj mob, tshwj xeeb tshaj yog hauv kev kuaj mob rau kev ntsuas kev ua haujlwm ntawm lub siab thiab nrhiav pom cov kab mob caj ces cuam tshuam nrog galactose metabolism.
