X-GAL CAS: 7240-90-6 Tus nqi tsim khoom

Kev Hloov Xim: X-Gal feem ntau tsis muaj xim tab sis, thaum hydrolysis los ntawm β-galactosidase, nws hloov xiav. Qhov kev hloov xim no tso cai rau kev pom thiab kev ntsuas ntawm β-galactosidase kev ua ub no.

Kev Tshawb Pom Gene LacZ: X-Gal yog siv los txheeb xyuas cov cell lossis cov qauv caj ces uas qhia txog lacZ gene. LacZ feem ntau yog siv ua tus reporter gene hauv molecular biology los ntsuam xyuas kev qhia txog gene lossis kawm txog kev ua haujlwm ntawm promoter.

Kev Tshuaj Xyuas Cov Kab Mob: X-Gal feem ntau siv rau hauv kev tshuaj xyuas cov kab mob bacterial. Cov kab mob bacterial uas muaj LacZ tshwm sim xiav thaum loj hlob ntawm agar uas muaj X-Gal, ua rau yooj yim txheeb xyuas thiab xaiv cov kab mob lacZ-positive.

Kev Tshawb Fawb Txog Kev Sib Koom Tes Gene: X-Gal kuj tseem siv rau hauv kev sim sib koom tes gene. Thaum lub hom phiaj gene txuas nrog lub lacZ gene, X-Gal staining tuaj yeem qhia tau tus qauv kev qhia tawm ntawm cov protein sib koom tes hauv lub cell lossis cov ntaub so ntswg.

Kev Tshawb Pom Cov Protein Hauv Qhov Chaw: X-Gal staining siv tau los tshawb nrhiav qhov chaw ntawm cov protein hauv subcellular. Los ntawm kev sib xyaw cov protein uas xav tau rau lub lacZ gene, qhov kev ua haujlwm β-galactosidase tuaj yeem qhia qhov chaw uas cov protein nyob hauv ib lub cell.

X-Gal Cov Khoom Siv Sib Piv: Cov qauv hloov kho ntawm X-Gal, xws li Bluo-Gal lossis Red-Gal, tau tsim los tso cai rau lwm txoj kev tsim xim. Cov khoom siv sib piv no ua rau muaj kev sib txawv ntawm cov hlwb lossis cov ntaub so ntswg lacZ-positive thiab lacZ-negative siv cov xim sib txawv.