4-NITROPHENYL-ALPH-D-MANNOPYRANOSIDE CAS TSIS TAU: 10357-27-4

Enzyme substrates: 4NPM tuaj yeem siv los ua lub substrate rau ntau yam enzymes, suav nrog glycosidases thiab lwm yam enzymes.Cov enzymes no tshem tawm glycosidic daim ntawv cog lus ntawm mannose thiab 4NPM, ua rau tso tawm ntawm nitrophenyl moiety.Qhov twg ntawm substrate hydrolysis tuaj yeem ntsuas spectrophotometrically los ntawm kev saib xyuas qhov nqus ntawm cov tshuaj nitrophenyl tso tawm ntawm ib lub wavelength tshwj xeeb.Qhov no tso cai rau cov kws tshawb fawb los soj ntsuam cov kev ua enzyme thiab kinetics.

Kev soj ntsuam rau cov metabolism hauv carbohydrate: Los ntawm kev siv 4NPM ua lub substrate, cov kws tshawb fawb tuaj yeem kawm txog cov haujlwm ntawm cov enzymes koom nrog hauv cov metabolism hauv carbohydrate, xws li alpha-mannosidases.Cov enzymes no hydrolyze glycosidic bonds nyob rau hauv mannose-muaj cov tebchaw, thiab lawv cov dej num tuaj yeem ntsuas los ntawm kev saib xyuas kev tso tawm ntawm nitrophenyl moiety los ntawm 4NPM.

Kev tshawb fawb glycosylation: 4NPM kuj tuaj yeem siv rau hauv kev tshuaj ntsuam xyuas cov enzymes koom nrog cov txheej txheem glycosylation.Glycosylation yog cov txheej txheem ntawm kev txuas cov piam thaj molecules rau cov proteins lossis lwm cov molecules, thiab ntau cov enzymes koom nrog hauv cov txheej txheem no.Los ntawm kev siv 4NPM ua ib qho kev lees txais substrate, cov kws tshawb fawb tuaj yeem ntsuas qhov hloov pauv ntawm cov piam thaj moiety rau 4NPM los ntawm cov enzymes tshwj xeeb koom nrog hauv cov tshuaj tiv thaiv glycosylation.

Kev tshuaj ntsuam xyuas enzyme inhibitors lossis activators: 4NPM tuaj yeem siv rau hauv kev tshuaj ntsuam xyuas siab los txheeb xyuas cov tebchaw uas inhibit lossis qhib cov enzymes tshwj xeeb.Los ntawm kev ntsuam xyuas cov txiaj ntsig ntawm cov tshuaj ntsuam xyuas ntawm hydrolysis lossis kev hloov pauv ntawm 4NPM los ntawm lub hom phiaj enzymes, cov kws tshawb fawb tuaj yeem txheeb xyuas cov neeg ua haujlwm kho mob lossis cov tshuaj muaj txiaj ntsig zoo rau kev kawm enzymes.