4-NITROPHENYL-ALPHA-D-MANNOPYRANOSIDE CAS: 10357-27-4

Cov khoom siv enzyme: 4NPM siv tau ua cov khoom siv rau ntau yam enzymes, suav nrog glycosidases thiab lwm yam enzymes. Cov enzymes no txiav cov glycosidic bond ntawm mannose thiab 4NPM, ua rau muaj kev tso tawm ntawm nitrophenyl moiety. Qhov dav ntawm cov substrate hydrolysis tuaj yeem ntsuas spectrophotometrically los ntawm kev saib xyuas qhov absorbance ntawm pawg nitrophenyl tso tawm ntawm ib qho wavelength tshwj xeeb. Qhov no tso cai rau cov kws tshawb fawb los ntsuas qhov kev ua haujlwm ntawm enzyme thiab kinetics.

Kev ntsuam xyuas rau kev ua haujlwm ntawm cov carbohydrate metabolism: Los ntawm kev siv 4NPM ua lub substrate, cov kws tshawb fawb tuaj yeem kawm txog kev ua haujlwm ntawm cov enzymes uas koom nrog kev ua haujlwm ntawm cov carbohydrate metabolism, xws li alpha-mannosidases. Cov enzymes no hydrolyze glycosidic bonds hauv cov tshuaj mannose-muaj, thiab lawv cov haujlwm tuaj yeem ntsuas los ntawm kev saib xyuas kev tso tawm ntawm nitrophenyl moiety los ntawm 4NPM.

Kev tshawb nrhiav Glycosylation: 4NPM kuj tseem siv tau rau hauv kev sim los tshawb nrhiav cov enzymes uas koom nrog cov txheej txheem glycosylation. Glycosylation yog cov txheej txheem ntawm kev txuas cov suab thaj rau cov protein lossis lwm cov molecules, thiab ntau cov enzymes koom nrog hauv cov txheej txheem no. Los ntawm kev siv 4NPM ua tus txais substrate, cov kws tshawb fawb tuaj yeem ntsuas qhov kev hloov pauv ntawm cov suab thaj rau 4NPM los ntawm cov enzymes tshwj xeeb uas koom nrog cov tshuaj tiv thaiv glycosylation.

Kev tshuaj xyuas cov tshuaj enzyme inhibitors lossis activators: 4NPM tuaj yeem siv rau hauv kev tshuaj ntsuam xyuas high-throughput los txheeb xyuas cov tshuaj uas inhibit lossis ua kom cov enzymes tshwj xeeb ua haujlwm. Los ntawm kev soj ntsuam cov nyhuv ntawm cov tshuaj kuaj rau hydrolysis lossis kev hloov kho ntawm 4NPM los ntawm cov enzymes lub hom phiaj, cov kws tshawb fawb tuaj yeem txheeb xyuas cov neeg sawv cev kho mob lossis cov tshuaj probes muaj txiaj ntsig rau kev kawm txog kev ua haujlwm ntawm enzyme.