5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide CAS: 4264-82-8

5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide (X-Gluc) yog ib qho chromogenic substrate uas feem ntau siv rau kev kuaj pom beta-glucuronidase (GUS) kev ua haujlwm. GUS yog ib qho enzyme pom muaj nyob rau hauv ntau yam kab mob, suav nrog cov kab mob me me, cov nroj tsuag, thiab cov tsiaj txhu. X-Gluc feem ntau siv rau hauv GUS reporter assays thiab kev tshawb fawb molecular biology.

Daim ntawv thov tseem ceeb ntawm X-Gluc yog nyob rau hauv cov txheej txheem histochemical staining, qhov twg nws tuaj yeem pom qhov kev qhia tawm thiab kev ua haujlwm ntawm GUS enzyme. Cov substrate no yog cell-permeable thiab dhau los ua hydrolyzed los ntawm GUS, ua rau muaj cov precipitate xiav lossis cov khoom tsis yaj. Cov xim xiav no tso cai rau cov kws tshawb fawb txheeb xyuas thiab nrhiav qhov chaw GUS kev ua haujlwm hauv cov cell, cov ntaub so ntswg, thiab tag nrho cov kab mob.

X-Gluc kuj tseem siv tau rau hauv kev ntsuas kom ntsuas tau GUS enzyme kev ua haujlwm. Qhov muaj zog ntawm cov xim xiav lossis qhov ntau ntawm cov khoom tsim tuaj yeem cuam tshuam nrog qib ntawm GUS kev qhia tawm lossis nws cov enzymatic kev ua haujlwm.

Tsis tas li ntawd xwb, X-Gluc tau siv rau hauv kev tshawb fawb txog noob caj noob ces ntawm cov nroj tsuag los kawm txog kev qhia tawm noob caj noob ces, kev ua haujlwm ntawm cov neeg txhawb nqa, thiab kev hloov pauv ntawm cov nroj tsuag. Nws kuj tau siv rau hauv cov kab mob bacterial rau kev cloning thiab nrhiav pom GUS fusion proteins.