Phenylgalactoside CAS: 2818-58-8

Cov nyhuv rau kev ua haujlwm ntawm enzyme: Phenylgalactoside feem ntau yog siv los ntsuas qhov kev ua haujlwm ntawm cov enzyme β-galactosidase. Thaum phenylgalactoside raug hydrolyzed los ntawm β-galactosidase, nws tso tawm p-nitrophenol. Kev sib sau ua ke ntawm p-nitrophenol tuaj yeem ntsuas tau ntau yam, muab kev nkag siab txog kev ua haujlwm ntawm β-galactosidase. Cov nyhuv no yog siv rau hauv cov ntawv thov xws li kev ntsuam xyuas enzyme thiab cov txheej txheem tshuaj ntsuam.

Kev tshuaj xyuas kev qhia tawm ntawm cov noob caj noob ces: Phenylgalactoside feem ntau siv ua lub hauv paus hauv kev sim tshuaj molecular biology los kawm txog kev qhia tawm ntawm cov noob caj noob ces. Lub noob caj noob ces lacZ, uas encodes β-galactosidase, feem ntau yog sib xyaw nrog cov kab ke tswj hwm ntawm lwm cov noob caj noob ces uas xav paub. Kev qhia tawm ntawm lub noob caj noob ces lacZ thiab kev hydrolysis ntawm phenylgalactoside los ntawm β-galactosidase tuaj yeem qhia txog tus qauv kev qhia tawm thiab qib ntawm cov noob caj noob ces uas tau kawm.

Cov txheej txheem tshuaj ntsuam: Phenylgalactoside yog ib qho tseem ceeb ntawm cov txheej txheem tshuaj ntsuam uas siv β-galactosidase kev ua haujlwm. Ib qho piv txwv uas paub dav yog txoj kev tshuaj ntsuam xiav-dawb, uas yog siv los txheeb xyuas cov hlwb recombinant lossis hloov pauv hauv kev sim molecular biology. Cov pawg uas tau txais cov recombinant DNA lossis tau dhau los ntawm kev sib xyaw ua ke ntawm cov noob caj noob ces yuav qhia β-galactosidase, ua rau hydrolysis ntawm phenylgalactoside thiab tsim cov xim xiav.

Kev ntxuav cov protein: Qee zaum, phenylgalactoside tuaj yeem siv ua ligand rau affinity chromatography los ntxuav cov protein uas khi rau lossis ua haujlwm los ntawm β-galactosidase. Cov protein uas xav tau yuav muaj daim ntawv lo affinity lossis daim ntawv lo fusion uas muaj β-galactosidase-binding domain. Los ntawm kev dhau cov protein sib xyaw los ntawm ib kem nrog immobilized phenylgalactoside, cov protein xav tau tuaj yeem khaws cia thiab tom qab ntawd eluted.