CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate) yog ib qho tshuaj ntxuav tes siv hauv biochemistry thiab molecular biology.Nws yog zwitterionic detergent, txhais tau tias nws muaj ob qho tib si zoo thiab tsis zoo them pawg.
CHAPS paub txog nws lub peev xwm los solubilize thiab stabilize membrane proteins, ua rau nws pab tau rau ntau yam kev siv xws li protein extraction, purification, thiab characterization.Nws cuam tshuam lipid-protein kev sib cuam tshuam, tso cai rau cov kua nplaum membrane kom muab rho tawm hauv lawv lub xeev.
Tsis zoo li lwm cov tshuaj detergents, CHAPS kuj yog me me thiab tsis denature feem ntau cov proteins, ua rau nws yog ib qho kev xaiv zoo heev rau kev tswj cov qauv protein thiab kev ua haujlwm thaum sim.Nws tseem tuaj yeem pab tiv thaiv kev sib sau ua ke ntawm cov protein.
CHAPS feem ntau siv rau hauv cov txheej txheem xws li SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis), isoelectric focusing, thiab Western blotting.Nws kuj tau nquag siv hauv cov kev tshawb fawb uas muaj cov enzymes membrane-bound, cov teeb liab hloov, thiab kev sib cuam tshuam ntawm cov protein-lipid.