TRIS-Acetate CAS: 6850-28-8 Tus nqi tsim khoom

Tris-acetate (TRIS-Acetate) yog ib qho buffer uas siv ntau hauv kev sim tshuaj lom neeg thiab biochemical. Nws muaj kev sib xyaw ua ke ntawm tris (hydroxymethyl) aminomethane (Tris) thiab acetic acid, uas ua haujlwm ua tus tswj pH thiab stabilizer. pH ntawm TRIS-Acetate buffer feem ntau yog txij li 7.4 txog 8.4.
Qhov cuam tshuam tseem ceeb ntawm TRIS-Acetate yog kom tswj tau pH kom ruaj khov, uas yog qhov tseem ceeb rau ntau yam kev hloov pauv ntawm lub cev thiab biochemical. Nws ua haujlwm ua ib qho buffer los ntawm kev txo qis txhua qhov kev hloov pauv tseem ceeb hauv pH uas yuav tshwm sim vim muaj cov kua qaub lossis cov hauv paus ntxiv thaum lub sijhawm sim.
TRIS-Acetate pom ntau yam kev siv hauv molecular biology, biochemistry, thiab biotechnology:
DNA thiab RNA Electrophoresis: TRIS-Acetate feem ntau siv ua ib qho buffer khiav hauv agarose thiab polyacrylamide gel electrophoresis. Nws muab ib puag ncig pH ruaj khov thaum lub sijhawm sib cais ntawm DNA thiab RNA fragments raws li lawv qhov loj me.
Kev Tshuaj Xyuas Cov Protein: TRIS-Acetate buffers yog siv rau cov protein electrophoresis, xws li SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis). Nws ua kom cov protein ruaj khov thiab sib cais thaum lub sijhawm ua haujlwm.
Cov Tshuaj Tiv Thaiv Enzyme: TRIS-Acetate buffers feem ntau siv rau hauv kev ntsuam xyuas thiab kev tshawb fawb enzyme. Nws muab qhov pH zoo tshaj plaws rau ntau yam tshuaj tiv thaiv enzymatic thiab pab tswj kev ua haujlwm ntawm enzyme.
Kev Cog Qoob Loo ntawm Lub Cev thiab Cov Nqaij: TRIS-Acetate buffers yog siv rau hauv cov khoom siv cog qoob loo ntawm lub cev kom tswj tau pH kom tsim nyog rau kev loj hlob ntawm lub cev thiab kev nthuav dav ntawm lub cev. Nws pab tswj cov xwm txheej ntawm lub cev uas xav tau rau kev muaj sia nyob ntawm lub cev.