Diammonium 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonate), feem ntau hu ua ABTS, yog ib qho uas nquag siv chromogenic substrate hauv biochemical ntsuam xyuas, tshwj xeeb tshaj yog nyob rau hauv lub teb ntawm enzymology.Nws yog cov khoom siv hluavtaws uas siv los ntsuas kev ua haujlwm ntawm ntau yam enzymes, suav nrog peroxidases thiab oxidases.
ABTS tsis muaj xim nyob rau hauv nws daim ntawv oxidized tab sis tig xiav-ntsuab thaum oxidized los ntawm ib qho enzyme nyob rau hauv muaj hydrogen peroxide los yog molecular oxygen.Qhov kev hloov xim no yog vim qhov tsim ntawm radical cation, uas absorbs lub teeb nyob rau hauv lub spectrum pom.
Cov tshuaj tiv thaiv ntawm ABTS thiab cov enzyme tsim cov khoom xim uas tuaj yeem ntsuas spectrophotometrically.Qhov kev siv ntawm cov xim yog ncaj qha proportional rau lub enzymatic kev ua si, tso cai rau cov kws tshawb fawb los soj ntsuam ntau npaum li cas enzyme kinetics, enzyme inhibition, los yog enzyme-substrate kev sib cuam tshuam.
ABTS muaj ntau yam kev siv hauv ntau yam, suav nrog kev kuaj mob, kev tshawb fawb tshuaj, thiab kev tshawb fawb txog zaub mov.Nws yog ib qho rhiab heev thiab muaj ntau yam dynamic ntau yam, ua rau nws yog ib qho kev xaiv nrov rau ntau qhov kev ntsuam xyuas biochemical.