Phenylgalactoside CAS: 2818-58-8

Cov txiaj ntsig ntawm kev ua enzyme: Phenylgalactoside feem ntau yog siv los ntsuas kev ua haujlwm ntawm enzyme β-galactosidase.Thaum phenylgalactoside yog hydrolyzed los ntawm β-galactosidase, nws tso p-nitrophenol.Kev sib sau ntawm p-nitrophenol tuaj yeem ntsuas qhov ntau, muab kev nkag siab txog kev ua haujlwm ntawm β-galactosidase.Cov nyhuv no yog siv rau hauv kev siv xws li kev tshuaj ntsuam enzyme thiab kev tshuaj ntsuam xyuas.

Kev tsom xam cov noob caj noob ces: Phenylgalactoside feem ntau yog siv los ua ib qho substrate hauv kev sim molecular biology los kawm txog cov noob caj noob ces.Lub lacZ noob, uas encodes β-galactosidase, feem ntau fused nrog kev tswj hwm ntawm lwm cov noob uas nyiam.Kev qhia ntawm lacZ gene thiab hydrolysis ntawm phenylgalactoside los ntawm β-galactosidase tuaj yeem qhia cov qauv qhia thiab qib ntawm cov hom phiaj tau kawm.

Kev tshuaj ntsuam xyuas: Phenylgalactoside yog ib qho tseem ceeb ntawm kev tshuaj ntsuam xyuas uas ua rau siv cov kev ua ntawm β-galactosidase.Ib qho piv txwv paub dav yog txoj kev tshuaj ntsuam xiav-dawb, uas yog siv los txheeb xyuas cov hlwb recombinant lossis hloov pauv hauv cov kev sim molecular biology.Cov cheeb tsam uas tau ua tiav cov DNA recombinant los yog cov noob caj noob ces recombination yuav qhia β-galactosidase, ua rau hydrolysis ntawm phenylgalactoside thiab tsim cov xim xiav.

Protein purification: Qee qhov xwm txheej, phenylgalactoside tuaj yeem siv los ua lub ligand rau affinity chromatography los ntxuav cov proteins uas tshwj xeeb khi rau lossis tau qhib los ntawm β-galactosidase.Cov protein ntawm kev txaus siab yuav muaj ib qho affinity tag lossis fusion tag uas muaj β-galactosidase-binding domain.Los ntawm kev hla cov protein sib tov los ntawm ib kab nrog immobilized phenylgalactoside, cov protein xav tau tuaj yeem xaiv khaws cia thiab tom qab eluted.