ONPG CAS: 369-07-3 Chaw tsim tshuaj paus Nqe

Cov nyhuv ntawm ONPG raws li lub substrate yog yuav tsum tau cleaved los ntawm lub enzyme β-galactosidase, uas ua rau tso tawm cov khoom daj, o-nitrophenol.Qhov kev hloov xim no tuaj yeem ntsuas spectrophotometrically, tso cai rau kom muaj nuj nqis ntawm β-galactosidase kev ua haujlwm.Cov ntawv thov ntawm ONPG feem ntau yog nyob rau hauv kev ntsuam xyuas cov noob qhia hauv molecular biology thiab microbiology tshawb fawb.Nws yog feem ntau siv los ntsuas β-galactosidase kev ua haujlwm raws li tus neeg sau xov xwm rau kev tshawb fawb txog noob caj noob ces, tshwj xeeb tshaj yog nyob rau hauv cov kab mob xws li E. coli.Lub lacZ gene, uas encodes β-galactosidase, feem ntau yog siv los ua tus cim rau kev tshuaj ntsuam gene qhia, raws li nws cov lus qhia tuaj yeem raug ntxias los ntawm cov xwm txheej tshwj xeeb lossis tswj los ntawm cov kws tshaj lij tshwj xeeb.Qhov kev ntsuam xyuas ONPG muab txoj hauv kev yooj yim thiab txhim khu kev qha los ntsuas qib ntawm gene qhia los ntawm kev ntsuas qhov kev ua ntawm β-galactosidase.Qhov kev ntsuam xyuas no tau siv dav hauv ntau daim ntawv thov, xws li kev kawm txhawb kev ua haujlwm, kev tswj cov noob, thiab kev sib cuam tshuam ntawm cov protein-protein.Tsis tas li ntawd, nws tuaj yeem siv los txiav txim siab enzyme kinetics thiab ntsuas qhov cuam tshuam ntawm kev hloov pauv lossis kev kho mob ntawm kev ua enzyme.