HEPES-Na CAS: 75277-39-3 Chaw tsim tshuaj paus Nqe

Buffering tus neeg saib xyuas: HEPES sodium ntsev feem ntau yog siv los ua tus neeg sawv cev hauv kev sim tshuaj lom neeg thiab biochemical.Nws pab tswj cov pH ruaj khov, tshwj xeeb tshaj yog nyob rau hauv physiological ntau yam (pH 7.2-7.6).Nws lub peev xwm buffering ua rau nws muaj txiaj ntsig hauv kev tswj xyuas cov xwm txheej zoo rau ntau yam kev cuam tshuam enzymatic, kab lis kev cai ntawm tes, thiab cov txheej txheem molecular biology.

Cell kab lis kev cai: HEPES sodium ntsev yog dav siv los ua tus neeg sawv cev hauv xov tooj ntawm tes.Kev muaj peev xwm tswj tau qhov pH ruaj khov yog qhov tseem ceeb rau kev loj hlob thiab muaj peev xwm ntawm cov hlwb.HEPES feem ntau nyiam dua lwm cov neeg ua haujlwm buffering vim nws tsis pom qhov hloov pauv tseem ceeb hauv pH thaum raug CO2 atmospheric.

Kev tshawb fawb enzyme: HEPES sodium ntsev yog qhov tshwj xeeb tshaj yog muaj txiaj ntsig hauv kev tshawb fawb enzymatic qhov twg yuav tsum muaj ib puag ncig pH tas li thiab tswj tau.Nws tiv thaiv kev hloov pauv tsis zoo ntawm pH thaum lub sij hawm cov tshuaj tiv thaiv enzymatic, kom ntseeg tau tias kev ua haujlwm zoo enzyme.

Electrophoresis: HEPES sodium ntsev feem ntau yog siv los ua tus neeg sawv cev hauv ntau cov txheej txheem electrophoretic xws li polyacrylamide gel electrophoresis (PAGE) thiab agarose gel electrophoresis.Nws pab tswj cov pH ntawm qhov tsis, uas yog qhov tseem ceeb rau kev sib cais thiab tshuaj xyuas nucleic acids thiab cov proteins.

Kev soj ntsuam biochemical: HEPES sodium ntsev feem ntau yog siv ntau yam kev tshuaj ntsuam xyuas biochemical, suav nrog kev soj ntsuam enzyme, tshuaj ntsuam xyuas cov protein ntau, thiab kev soj ntsuam spectrophotometric.Nws pab tswj hwm qhov xav tau pH ntau yam uas xav tau kom raug thiab rov tsim dua.

Kev tsim tshuaj: HEPES sodium ntsev kuj siv rau hauv cov tshuaj formulations ua tus neeg sawv cev buffering kom stabilize cov tshuaj formulations thiab tswj cov pH yam xav tau.