HEPES-Na CAS:75277-39-3 Tus nqi tsim khoom

Tus neeg sawv cev buffering: HEPES sodium ntsev feem ntau yog siv los ua tus neeg sawv cev buffering hauv kev sim tshuaj lom neeg thiab biochemical. Nws pab tswj kom muaj pH ruaj khov, tshwj xeeb tshaj yog nyob rau hauv qhov ntau ntawm physiological (pH 7.2-7.6). Nws lub peev xwm buffering ua rau nws muaj txiaj ntsig zoo hauv kev tswj hwm cov xwm txheej zoo rau ntau yam kev cuam tshuam enzymatic, kev cog qoob loo ntawm tes, thiab cov txheej txheem molecular biology.

Kev cog qoob loo ntawm tes: HEPES sodium ntsev yog siv dav ua tus neeg sawv cev buffering hauv cov xov xwm cog qoob loo ntawm tes. Lub peev xwm los tswj kom muaj pH ruaj khov yog qhov tseem ceeb rau kev loj hlob thiab kev muaj sia nyob ntawm cov tes. HEPES feem ntau nyiam dua lwm cov neeg sawv cev buffering vim nws tsis qhia txog kev hloov pauv tseem ceeb hauv pH thaum raug rau CO2 huab cua.

Kev tshawb fawb txog enzyme: HEPES sodium ntsev yog qhov tshwj xeeb tshaj yog pab tau rau kev tshawb fawb txog enzymatic qhov twg xav tau ib puag ncig pH tas mus li thiab tswj tau. Nws tiv thaiv kev hloov pauv loj heev hauv pH thaum lub sijhawm enzymatic reactions, ua kom muaj kev ua haujlwm zoo tshaj plaws ntawm enzyme.

Electrophoresis: HEPES sodium ntsev feem ntau yog siv los ua tus neeg sawv cev buffering hauv ntau yam txheej txheem electrophoretic xws li polyacrylamide gel electrophoresis (PAGE) thiab agarose gel electrophoresis. Nws pab tswj pH ntawm lub buffer, uas yog qhov tseem ceeb rau kev sib cais thiab kev soj ntsuam nucleic acids thiab cov protein.

Kev ntsuam xyuas biochemical: HEPES sodium ntsev feem ntau siv rau hauv ntau yam kev ntsuam xyuas biochemical, suav nrog kev ntsuam xyuas enzyme, kev ntsuam xyuas protein, thiab kev ntsuam xyuas spectrophotometric. Nws pab tswj qhov pH xav tau rau cov txiaj ntsig raug thiab rov ua dua.

Cov tshuaj tsim: HEPES sodium ntsev kuj tseem siv rau hauv cov tshuaj tsim ua tus neeg sawv cev buffering kom ruaj khov cov tshuaj tsim thiab tswj cov pH xav tau.