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Vancomycin hydrochloride CAS: 1404-93-9
Vancomycin hydrochloride yog ib hom tshuaj tua kab mob uas siv los kho cov kab mob hnyav uas tshwm sim los ntawm qee hom kab mob bacteria. Nws yog ib hom tshuaj glycopeptide thiab ua haujlwm los ntawm kev txwv tsis pub tsim cov peptidoglycan, ib qho tseem ceeb ntawm cov phab ntsa ntawm cov kab mob bacteria. Nws feem ntau siv los kho cov kab mob xws li MRSA, Clostridium difficile-associated diarrhea, thiab bacterial endocarditis..
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HEPPS CAS:16052-06-5 Tus nqi tsim khoom
HEPPS, uas yog sawv cev rau N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic acid, yog ib qho zwitterionic buffering agent uas feem ntau siv rau hauv kev tshawb fawb txog tsiaj txhu thiab biochemical. Nws paub txog nws lub peev xwm buffering zoo heev thiab ruaj khov ntawm ntau yam pH, tshwj xeeb tshaj yog nyob rau hauv ntau yam pH 6.8-8.2. HEPPS feem ntau siv rau hauv cov khoom siv kab lis kev cai ntawm tes, kev ntxuav cov protein, cov tshuaj enzymatic, thiab lwm yam kev siv uas xav tau ib puag ncig pH uas tswj tau zoo. Nws yaj tau hauv dej thiab feem ntau siv rau ntawm qhov concentration ntawm 10 mM txog 100 mM. HEPPS suav tias tsis muaj tshuaj lom rau tes thiab muaj kev cuam tshuam tsawg, ua rau nws yog ib qho kev xaiv nrov rau ntau cov txheej txheem sim.
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2,3,5-Triphenyl-2H-tetrazolium chloride CAS: 298-96-4
2,3,5-Triphenyl-2H-tetrazolium chloride, tseem hu ua TTC, yog ib qho tshuaj uas feem ntau siv ua tus qhia redox hauv kev siv tshuaj lom neeg thiab tshuaj lom neeg. Nws zoo li hmoov crystalline daj-dawb.
TTC feem ntau siv rau hauv ntau yam kev sim tshuaj lom neeg thiab kev sim los txiav txim siab txog kev muaj sia nyob ntawm cov cell thiab kev ua haujlwm ntawm cov metabolism. Thaum ntxiv rau cov cell uas muaj sia nyob, nws raug txo kom tsawg los ua cov khoom formazan liab. Qhov kev txo qis no yog nyob ntawm cov enzymes dehydrogenase uas muaj nyob hauv cov cell uas muaj sia nyob. Yog li ntawd, qhov ntau ntawm cov formazan liab uas tsim tawm yog sib npaug rau tus lej ntawm cov cell uas muaj sia nyob ntawm cov metabolism.
TTC lub peev xwm los ntsuam xyuas qhov muaj sia nyob ntawm cov cell ua rau nws muaj txiaj ntsig zoo rau kev tshawb fawb xws li kev kuaj cytotoxicity, kev ntsuam xyuas cell proliferation, thiab kev ntsuam xyuas qhov ua tau zoo ntawm cov tshuaj. Tsis tas li ntawd, nws siv rau hauv microbiology rau kev ntsuam xyuas kev loj hlob ntawm cov kab mob, nrog rau kev tshawb fawb txog kev cog qoob loo thiab kev ntsuam xyuas qhov muaj sia nyob ntawm cov ntaub so ntswg.
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Gly-Pro p-nitroanilide p-toluenesulfonate ntsev CAS: 65096-46-0
Gly-Pro p-nitroanilide p-toluenesulfonate ntsev yog ib qho tshuaj siv hauv kev ntsuas enzymatic los ntsuas qhov kev ua haujlwm ntawm proline-specific peptidases. Nws muaj cov dipeptide Gly-Pro txuas nrog pawg p-nitroanilide, uas tuaj yeem tso tawm los ntawm kev ua ntawm proline-specific peptidases.
Hauv kev ntsuam xyuas enzymatic, cov tshuaj no raug ntxiv rau hauv cov qauv uas muaj cov enzyme uas xav tau. Cov enzyme txiav cov kev sib txuas ntawm glycine (Gly) thiab proline (Pro), tso tawm pawg p-nitroanilide. Cov p-nitroanilide uas tso tawm tuaj yeem ntsuas spectrophotometrically, tso cai rau kev txiav txim siab ntawm cov enzyme ua haujlwm.
Cov p-toluenesulfonate ntsev ntawm Gly-Pro p-nitroanilide muab kev ruaj khov thiab solubility hauv cov kua dej, ua kom yooj yim rau nws siv hauv kev ntsuam xyuas enzymatic..
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5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide CAS: 4264-82-8
5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide yog ib qho tshuaj siv rau hauv ntau yam kev tshawb fawb biochemical, tshwj xeeb tshaj yog rau kev kuaj pom thiab pom kev ua haujlwm ntawm cov enzyme. Nws yog ib qho substrate uas tuaj yeem hydrolyzed los ntawm cov enzymes tshwj xeeb, ua rau muaj kev tso tawm ntawm cov khoom muaj xim lossis fluorescent.
Cov tshuaj no feem ntau siv rau hauv kev sim los ntes qhov muaj thiab kev ua haujlwm ntawm cov enzymes xws li beta-galactosidase thiab beta-glucuronidase. Cov enzymes no txiav cov acetyl thiab glucosaminide pawg ntawm cov substrate, ua rau muaj cov xim xiav lossis ntsuab chromophore.
Cov qauv tshwj xeeb ntawm 5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide ua rau yooj yim nrhiav thiab ntsuas qhov ua haujlwm ntawm cov enzyme. Nws siv rau hauv ntau txoj kev sim, suav nrog histochemistry, immunohistochemistry, thiab cell-based assays, tau pab txhawb rau kev nkag siab zoo dua ntawm cov haujlwm enzyme.
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AMPPD CAS:122341-56-4 Tus nqi tsim khoom
AMPPD, luv luv rau 3-(2′-spiroadamantane)-4-methoxy-4-(3″-phosphoryloxy)phenyl-1,2-dioxetane, yog ib qho chemiluminescent substrate uas feem ntau siv rau hauv kev siv biological thiab immunoassay. Nws yog siv dav hauv kev tshawb fawb molecular biology, kev kuaj mob, thiab kev tshawb fawb txog lub neej.
AMPPD yog tsim tshwj xeeb los siv ua ke nrog alkaline phosphatase enzyme conjugates. Thaum raug alkaline phosphatase, AMPPD dhau los ua qhov kev sib cais enzymatic sai thiab ua haujlwm tau zoo, ua rau muaj lub teeb ci.
Lub teeb uas tawm los ntawm AMPPD raug ntes los ntawm kev siv cov tshuab thaij duab rhiab heev lossis luminometers, uas tso cai rau kev ntsuas thiab pom cov molecule lossis analytes uas xav tau. Txoj kev ntes raws li chemiluminescence no muaj kev rhiab heev, suab nrov qis, thiab ntau yam dynamic.
Vim nws ruaj khov thiab lub teeb liab ntev, AMPPD zoo rau cov ntawv thov xws li Western blotting, ELISA (enzyme-linked immunosorbent assay), DNA hybridization, thiab in situ hybridization. Nws ua rau cov kws tshawb fawb nrhiav thiab tshuaj xyuas cov protein, nucleic acids, antibodies, thiab lwm yam biomolecules.
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2,6-Dichloroindophenol sodium ntsev CAS: 620-45-1
3,5-Dichlorophenyldiazonium tetrafluoroborate yog ib yam tshuaj uas feem ntau siv rau hauv kev tsim cov organic. Nws yog ib qho ntsev diazonium, uas txhais tau tias nws muaj ib pawg diazonium (N2+) txuas rau lub nplhaib phenyl uas tau hloov nrog cov chlorine atoms ntawm qhov chaw thib 3 thiab thib 5.
Cov tshuaj no feem ntau siv rau hauv cov tshuaj tiv thaiv uas cuam tshuam nrog kev sib txuas nrog lwm cov tshuaj aromatic. Nws tuaj yeem dhau los ntawm cov tshuaj tiv thaiv xws li electrophilic aromatic substitution, qhov twg nws tuaj yeem hloov nws pawg diazonium mus rau lwm cov tshuaj nucleophilic aromatic los tsim ib daim ntawv cog lus tshiab. Qhov no yog qhov tshwj xeeb tshaj yog rau kev qhia txog cov pab pawg ua haujlwm rau ntawm cov nplhaib aromatic..
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3,3′-Diaminobenzidine CAS: 91-95-2
3,3′-Diaminobenzidine (DAB) yog ib hom tshuaj uas siv ntau hauv kev siv immunohistochemistry (IHC) thiab immunocytochemistry (ICC). Nws yog ib qho chromogenic substrate uas tsim cov khoom xim av thaum raug rau peroxidase enzymes, qhia tias muaj cov antigen uas xav tau.
DAB feem ntau siv los pom cov protein lossis lwm yam biomolecules uas xav paub hauv cov qauv tsiaj txhu, xws li cov nqaij lossis cov cell. Nws tuaj yeem muab ib qho cim qhia rau thaj chaw thiab pom tseeb rau lub hom phiaj antigen, uas tso cai rau cov kws tshawb fawb txheeb xyuas thiab tshuaj xyuas nws qhov kev faib tawm thiab kev muaj ntau.
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(±)-6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid CAS: 53188-07-1
Trolox yog ib qho tshuaj sib xyaw ua ke uas yog ib feem ntawm cov tshuaj antioxidants hu ua vitamin E analogs. Nws yog ib qho tshuaj antioxidant muaj zog uas tuaj yeem tshem tawm cov free radicals thiab tiv thaiv cov cell thiab cov nqaij ntawm kev puas tsuaj oxidative. Trolox tau raug kawm txog nws cov txiaj ntsig kev noj qab haus huv, suav nrog nws lub peev xwm los txo qhov o, tiv thaiv kev puas tsuaj ntawm cell, thiab txhawb kev noj qab haus huv ntawm lub plawv. Nws feem ntau siv ua cov tshuaj siv hauv kev tshawb fawb txog antioxidant thiab ua cov khoom ntxiv rau hauv ntau yam khoom noj thiab cov khoom siv pleev kom zoo nkauj.
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1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride CAS:25952-53-8
1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, tseem hu ua EDC hydrochloride lossis EDAC, yog ib qho tshuaj lom neeg uas feem ntau siv hauv biochemistry thiab molecular biology ua tus neeg sawv cev rau kev ua kom cov pab pawg carboxyl ua haujlwm. Nws yog ib qho hmoov dawb crystalline uas yaj tau zoo heev hauv dej.
EDC hydrochloride feem ntau yog siv rau hauv kev tsim cov peptides thiab cov protein los ntawm kev pab txhawb kev tsim cov amide bonds ntawm carboxyl thiab amino pawg. Nws ua haujlwm los ntawm kev ua kom cov carboxyl pawg, uas cuam tshuam nrog cov amino pawg nyob rau hauv lub xub ntiag ntawm tus neeg sawv cev coupling xws li N-hydroxysuccinimide (NHS). Qhov kev tshuaj tiv thaiv no ua rau muaj kev tsim cov amide bonds ruaj khov.
EDC hydrochloride kuj tseem siv rau hauv kev tshawb fawb DNA thiab RNA rau kev hloov kho thiab sib txuas cov nucleic acids, nrog rau kev ua kom cov enzymes thiab lwm yam biomolecules tsis txav mus rau ntawm qhov chaw. Nws yog ib qho reagent uas muaj ntau yam uas tuaj yeem txhim kho qhov ua tau zoo thiab qhov tshwj xeeb ntawm cov tshuaj tiv thaiv no.
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HEPES CAS: 7365-45-9 Tus nqi tsim khoom
HEPES (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid) yog ib qho tshuaj buffer uas feem ntau siv rau hauv kev tshawb fawb txog kab mob thiab tshuaj lom neeg. Nws paub txog nws lub peev xwm los tswj kom muaj pH ruaj khov hauv cov kua dej, ua rau nws haum rau ntau yam kev siv uas cuam tshuam nrog cov txheej txheem cellular thiab enzymatic. HEPES yog ib qho zwitterionic compound, txhais tau tias nws muaj cov nqi zoo thiab tsis zoo ntawm cov pab pawg ua haujlwm sib cais, uas tso cai rau nws tiv taus kev hloov pauv hauv pH los ntawm kev ntxiv cov kua qaub lossis cov hauv paus. Nws feem ntau siv rau hauv kev cog qoob loo ntawm tes, kev ntsuam xyuas enzyme, kev kawm protein, kev sim electrophoresis, thiab cov qauv tshuaj.
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p-Nitrophenyl -aL-Fucopyranoside CAS: 10231-84-2
p-Nitrophenyl-α-L-Fucopyranoside yog ib qho tshuaj lom neeg uas yog ib feem ntawm nitrophenyl glycosides. Nws feem ntau siv rau hauv kev tshawb fawb biochemical ua lub substrate rau enzyme α-L-fucosidase, uas catalyzes nws hydrolysis. Cov tshuaj no tsis muaj xim thiab soluble hauv cov organic solvents, ua rau nws yooj yim rau kev ntsuam xyuas enzymatic.
