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PNPG CAS: 3150-24-1 Chaw tsim tshuaj paus Nqe
PNPG, los yog p-nitrophenyl β-D-glucopyranoside, yog ib qho me me molecule substrate feem ntau siv rau hauv biochemical ntsuam xyuas los ntsuas qhov kev ua ntawm glucosidase enzymes.Nws yog xim tsis muaj xim thiab tsis muaj fluorescent, tab sis raws li hydrolysis los ntawm glucosidase, nws hloov mus rau p-nitrophenol, uas muaj xim daj thiab pom tau yooj yim spectrophotometrically.
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ONPG CAS: 369-07-3 Chaw tsim tshuaj paus Nqe
O-nitrophenyl-β-D-galactopyranoside (ONPG) yog ib qho hluavtaws substrate siv nyob rau hauv biochemical thiab molecular biology tshuaj ntsuam xyuas kev ua ub no ntawm enzyme β-galactosidase.Nws yog feem ntau siv rau hauv kev tshuaj ntsuam xyuas cov noob qhia hauv cov kab mob, xws li Escherichia coli.ONPG yog cov xim tsis muaj xim uas yog cleaved los ntawm β-galactosidase, uas ua rau tso tawm daj compound, o-nitrophenol.Cov xim daj uas tsim tawm tuaj yeem ntsuas spectrophotometrically, muab kev ntsuas tsis ncaj ntawm cov enzyme kev ua haujlwm.Qhov kev ntsuam xyuas siv ONPG feem ntau hu ua ONPG kev soj ntsuam thiab yog ib txoj hauv kev siv dav hauv kev tshawb fawb molecular biology los ntsuas qhov qhia theem ntawm cov noob tswj los ntawm lac. operon nyob rau hauv cov kab mob hlwb.
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Nitrotetrazolium Blue Chloride CAS: 298-83-9
Nitrotetrazolium Blue Chloride (NBT) yog ib qho kev qhia redox feem ntau siv hauv kev tshuaj lom neeg thiab tshuaj lom neeg.Nws yog cov hmoov daj daj uas tig xiav thaum txo qis, ua rau nws muaj txiaj ntsig zoo rau kev kuaj pom muaj qee yam enzymes thiab kev ua haujlwm metabolic.
NBT reacts nrog cov khoom siv hluav taws xob thiab cov enzymes xws li dehydrogenases, uas koom nrog ntau yam txheej txheem ntawm tes.Thaum NBT raug txo los ntawm cov enzymes no, nws tsim ib qho xiav formazan precipitate, tso cai rau pom kev pom lossis spectrophotometric.
Cov tshuaj reagent no feem ntau siv rau hauv kev ntsuam xyuas xws li kev sim txo NBT, qhov twg nws yog siv los ntsuas qhov ua pa tawg ntawm lub cev tiv thaiv kab mob.Nws tseem tuaj yeem siv los kawm txog cov haujlwm enzyme thiab cov txheej txheem metabolic hauv kev tshawb fawb txog oxidative kev nyuaj siab, cell viability, thiab cell sib txawv.
NBT tau pom cov ntawv thov hauv ntau yam, suav nrog microbiology, immunology, thiab cell biology.Nws yog ntau yam, kuj ruaj khov, thiab siv tau yooj yim, ua rau nws yog ib qho kev xaiv nrov rau ntau cov txheej txheem sim.
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Neocuproine CAS: 484-11-7 Chaw tsim tshuaj paus Nqe
Neocuproine yog ib tus neeg sawv cev chelate uas feem ntau siv rau hauv ntau daim ntawv thov, suav nrog tshuaj chemistry thiab tshuaj.Nws muaj kev sib raug zoo rau tooj liab ions thiab tsim cov complexes ruaj khov nrog lawv.Cov cuab yeej no ua rau neocuproine muaj txiaj ntsig rau kev kuaj xyuas thiab ntsuas cov tooj liab hauv cov kev daws teeb meem lossis cov qauv.Tsis tas li ntawd, neocuproine tau kawm txog nws cov kev siv tshuaj kho mob, tshwj xeeb hauv kev kho mob qog noj ntshav thiab cov kab mob neurodegenerative.
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IPTG CAS: 367-93-1 Chaw tsim tshuaj paus Nqe
Isopropyl β-D-1-thiogalactopyranoside (IPTG) yog ib qho hluavtaws analogue ntawm lactose uas feem ntau siv rau hauv kev tshawb fawb molecular biology thiab kev siv biotechnology.IPTG feem ntau yog siv los txhawb kev qhia cov noob hauv cov kab mob hauv cov kab mob, qhov chaw uas nws ua haujlwm los ua cov tshuaj molecular los pib qhov kev hloov pauv ntawm cov hom phiaj.
Thaum ntxiv rau qhov kev loj hlob nruab nrab, IPTG yog coj los ntawm cov kab mob thiab tuaj yeem khi rau cov protein lac repressor, tiv thaiv nws los ntawm kev thaiv kev ua haujlwm ntawm lac operon.Lub lac operon yog ib pawg ntawm cov noob koom nrog hauv cov metabolism hauv lactose, thiab thaum tshem tawm cov protein repressor, cov noob tau nthuav tawm.
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HATU CAS: 148893-10-1 Chaw tsim tshuaj paus Nqe
HATU (1-[bis (dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxid hexafluorophosphate) yog ib qho kev sib txuas reagent hauv peptide synthesis thiab organic chemistry.
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D-fucose CAS: 3615-37-0 Chaw tsim tshuaj paus Nqe
D-fucose yog ib qho monosaccharide, tshwj xeeb tshaj yog rau 6-carbon qab zib, uas belongs rau pawg ntawm cov suab thaj yooj yim hu ua hexoses.Nws yog ib qho isomer ntawm qabzib, sib txawv hauv kev teeb tsa ntawm ib pawg hydroxyl.
D-fucose yog ib txwm muaj nyob rau hauv ntau yam kab mob, nrog rau cov kab mob, fungi, nroj tsuag, thiab tsiaj txhu.Nws ua lub luag haujlwm tseem ceeb hauv ntau cov txheej txheem lom neeg, xws li kev taw qhia ntawm tes, cell adhesion, thiab glycoprotein synthesis.Nws yog ib feem ntawm glycolipids, glycoproteins, thiab proteoglycans, uas muaj kev koom tes hauv kev sib txuas lus ntawm tes thiab kev paub.
Hauv tib neeg, D-fucose tseem koom nrog hauv biosynthesis ntawm cov qauv glycan tseem ceeb, xws li Lewis antigens thiab cov pab pawg ntshav antigens, uas muaj feem cuam tshuam rau kev sib xyaw ntawm cov ntshav thiab kev tiv thaiv kab mob.
D-fucose tuaj yeem tau txais los ntawm ntau qhov chaw, suav nrog seaweed, nroj tsuag, thiab microbial fermentation.Nws yog siv nyob rau hauv kev tshawb fawb thiab biomedical daim ntaub ntawv, raws li zoo raws li nyob rau hauv zus tau tej cov pharmaceuticals thiab kho tebchaw.
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DDT CAS: 3483-12-3 Chaw tsim tshuaj paus Nqe
DL-Dithiothreitol, tseem hu ua DTT, yog tus neeg sawv cev txo qis feem ntau siv hauv kev tshawb fawb biochemical thiab molecular biology.Nws yog ib qho me me molecule nrog ib pawg thiol (sulfur-muaj) ntawm txhua qhov kawg.
DTT feem ntau siv los rhuav tshem cov ntawv cog lus disulfide hauv cov proteins, uas yuav pab nthuav tawm lossis ua rau lawv.Qhov kev txo qis ntawm disulfide bonds yog qhov tseem ceeb hauv ntau cov txheej txheem kuaj xws li kev ua kom huv ntawm cov protein, gel electrophoresis, thiab kev tshawb fawb cov qauv protein.DTT kuj tseem siv tau los tiv thaiv thiol pawg thiab tiv thaiv oxidation thaum lub sijhawm sim.
DTT feem ntau yog ntxiv rau cov kev sim daws teeb meem hauv cov ntsiab lus me me, thiab nws txoj haujlwm yog nyob ntawm qhov muaj oxygen.Nws yog ib qho tseem ceeb uas yuav tsum tau ua tib zoo saib xyuas DTT vim nws muaj kev nkag siab rau huab cua, cua sov, thiab noo noo, uas tuaj yeem txo nws cov txiaj ntsig.
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D-(+)-Galactose CAS: 59-23-4 Chaw tsim tshuaj paus Nqe
D-(+)-Galactose yog cov suab thaj monosaccharide thiab ib qho tseem ceeb ntawm ntau cov txheej txheem lom neeg.Nws yog ib qho qab zib uas tshwm sim nyob rau hauv ntau yam khoom noj, xws li txiv hmab txiv ntoo, khoom noj siv mis, thiab zaub.
Galactose feem ntau metabolized nyob rau hauv lub cev los ntawm ib tug series ntawm enzymatic tshuaj.Nws ua lub luag haujlwm tseem ceeb hauv kev sib txuas lus ntawm tes, kev tsim hluav taws xob, thiab biosynthesis ntawm cov molecules tseem ceeb xws li glycolipids, glycoproteins, thiab lactose.
Nyob rau hauv cov nqe lus ntawm nws daim ntawv thov, D-(+)-Galactose yog feem ntau siv nyob rau hauv microbiology thiab biotechnology raws li ib tug carbon qhov chaw nyob rau hauv kab lis kev cai xov xwm rau kev loj hlob ntawm ntau yam kab mob.Nws kuj tseem siv rau hauv kev tsim ntau yam bioactive compounds, tshuaj, thiab khoom noj khoom haus.Tsis tas li ntawd, nws tau nquag siv los ua tus neeg saib xyuas kev kho mob, tshwj xeeb tshaj yog nyob rau hauv kev ntsuam xyuas rau kev soj ntsuam lub siab ua haujlwm thiab kuaj cov kab mob caj ces cuam tshuam nrog galactose metabolism.
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beta-D-Galactose pentaacetate CAS: 4163-60-4
Beta-D-Galactose pentaacetate yog ib qho tshuaj sib xyaw ua ke los ntawm galactose, monosaccharide qab zib.Nws yog tsim los ntawm acetylating txhua pawg hydroxyl ntawm galactose molecule nrog tsib pawg acetyl.
Cov tshuaj no feem ntau siv los ua tus neeg sawv cev tiv thaiv galactose hauv ntau yam tshuaj lom neeg thiab cov txheej txheem hluavtaws.Daim ntawv pentaacetate pab kom ruaj khov galactose thiab tiv thaiv kev tsis xav tau lossis hloov pauv thaum muaj kev cuam tshuam.
Tsis tas li ntawd, qhov sib xyaw no tuaj yeem siv los ua tus thawj coj rau kev sib txuas ntawm lwm cov galactose derivatives.Cov pab pawg acetyl tuaj yeem raug xaiv tshem tawm kom tau txais cov galactose derivatives sib txawv nrog cov pab pawg tshwj xeeb.
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5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium ntsev CAS: 129541-41-9
5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium ntsev yog cov tshuaj lom neeg feem ntau siv hauv kev tshawb nrhiav thiab kuaj mob.Nws yog feem ntau hu ua X-Gluc thiab tau dav siv los ua lub substrate rau kev tshawb pom ntawm beta-glucuronidase enzyme kev ua.
Thaum muaj beta-glucuronidase, nws cleaves glucuronide daim ntawv cog lus nyob rau hauv X-Gluc, ua rau lub liberation ntawm ib tug xiav dye hu ua 5-bromo-4-chloro-3-indolyl.Cov tshuaj tiv thaiv no feem ntau yog siv los pom qhov muag lossis spectrophotometrically kuaj pom qhov qhia ntawm beta-glucuronidase enzyme hauv cov hlwb lossis cov ntaub so ntswg.
Cov sodium ntsev daim ntawv ntawm X-Gluc txhim kho nws cov solubility nyob rau hauv aqueous daws, yooj yim rau nws siv nyob rau hauv kev soj ntsuam kuaj.X-Gluc feem ntau yog siv rau hauv kev tshawb fawb molecular biology los kawm txog cov noob caj noob ces, kev txhawb nqa kev ua haujlwm, thiab kev soj ntsuam gene.Nws tseem tuaj yeem siv los txheeb xyuas qhov muaj cov kab mob beta-glucuronidase-ua cov kab mob, xws li qee cov kab mob, hauv kev tshawb fawb microbiological.
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4-Nitrophenyl-beta-D-xylopyranoside CAS: 2001-96-9
4-Nitrophenyl-beta-D-xylopyranoside yog chromogenic substrate siv nyob rau hauv enzymatic assays los xyuas thiab ntsuas cov kev ua ntawm enzymes hu ua beta-xylosidases.
