BCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine ntsev CAS: 6578-06-9

BCIP-Toluidine, tseem hu ua 5-Bromo-4-chloro-3-indolylphosphate-p-toluidine ntsev, yog ib qho tshuaj lom neeg uas feem ntau siv rau hauv kev tshawb fawb txog molecular biology thiab biochemistry. Nws feem ntau yog siv ua lub substrate hauv enzyme-linked immunosorbent assays (ELISAs) lossis immunohistochemical staining techniques los ntes alkaline phosphatase kev ua haujlwm.

Thaum BCIP-Toluidine raug alkaline phosphatase txiav, nws dhau los ua qhov kev hloov pauv xim, tsim cov xim xiav-ntshav. Qhov kev tshuaj tiv thaiv no tso cai rau kev pom thiab kev ntsuas ntawm alkaline phosphatase kev ua ub no hauv cov qauv. Qhov muaj zog ntawm cov xim tuaj yeem ntsuas spectrophotometrically thiab yog ncaj qha proportional rau qhov kev ua ub no ntawm enzyme.

Kev siv BCIP-Toluidine ua ib qho substrate yog qhov tshwj xeeb tshaj yog nyob rau hauv cov ntawv thov uas muaj DNA lossis RNA labeling, xws li in situ hybridization (ISH) lossis Southern thiab Northern blotting. Nws muab ib txoj kev txhim khu kev qha thiab rhiab heev rau kev ntes qhov muaj cov nucleic acids hauv cov qauv.