8-Anilino-1-naphthalenesulfonic acid CAS: 82-76-8

8-Anilino-1-naphthalenesulfonic acid, tseem hu ua ANS, yog ib hom xim fluorescent uas feem ntau siv rau hauv kev tshawb fawb biochemical thiab biophysical. Nws yog ib hom organic compounds uas hu ua aniline-based sulfonic acids. ANS paub txog nws lub peev xwm los khi rau cov cheeb tsam hydrophobic lossis cov chaw hydrophobic uas raug rau hauv cov protein thiab lwm yam biomolecules.

ANS fluoresces thaum nws khi rau thaj chaw hydrophobic, ua rau nws yog ib qho kev soj ntsuam zoo rau kev kawm txog kev hloov pauv ntawm cov protein, kev sib cuam tshuam ntawm cov protein-protein, thiab cov txheej txheem folding/unfolding ntawm cov protein. Kev khi ntawm ANS rau thaj chaw hydrophobic ua rau muaj kev hloov pauv xiav hauv cov spectrum emission, uas tuaj yeem saib xyuas siv fluorescence spectroscopy.

ANS feem ntau siv rau hauv ntau yam kev tshawb fawb, suav nrog fluorescence polarization, fluorescence resonance energy transfer (FRET), thiab thermal stability assays. Nws tau siv los kawm txog cov qauv thiab kev ruaj khov ntawm cov protein, kev sib cuam tshuam ntawm cov protein-ligand, thiab cov txheej txheem sib sau ua ke ntawm cov protein.

Txiav txim los yog Thov Kev Hais Nqe

Cov Lus Qhia Txog Khoom

Cov Cim Npe Khoom

Daim Ntawv Thov thiab Cov Nyhuv

Fluorescent probe: ANS yog siv dav ua fluorescent probe los kawm txog kev hloov pauv ntawm cov protein, cov txheej txheem folding/unfolding protein, thiab kev sib cuam tshuam ntawm cov protein-ligand. Nws khi rau cov cheeb tsam hydrophobic lossis cov chaw hydrophobic uas raug rau hauv cov protein, ua rau muaj kev tso tawm fluorescence uas tuaj yeem saib xyuas siv fluorescence spectroscopy.

Kev kuaj pom hydrophobicity: ANS rhiab heev rau kev hloov pauv ntawm hydrophobicity ntawm qhov chaw ib puag ncig. Nws tuaj yeem siv los ntes thiab ntsuas surfactants, detergents, thiab lwm yam hydrophobic substances.

Kev ntsuam xyuas kev ruaj khov ntawm cov protein: ANS feem ntau siv rau hauv kev ntsuas kev ruaj khov ntawm cov protein. Los ntawm kev saib xyuas qhov muaj zog ntawm cov protein ANS uas khi rau hauv qab qhov kub sib txawv, nws muaj peev xwm txiav txim siab txog cov yam ntxwv ntawm kev hloov pauv ntawm cov protein thiab qhov kub yaj (Tm) ntawm cov protein.

Kev tshawb fawb txog kev sib sau ua ke ntawm cov protein: ANS siv tau los kawm txog cov txheej txheem sib sau ua ke ntawm cov protein, xws li kev tsim cov amyloid fibrils. Cov xim zas no rhiab heev rau cov cheeb tsam hydrophobic uas raug rau hauv cov protein sib sau ua ke, ua rau nws yog ib qho cuab yeej muaj txiaj ntsig zoo rau kev saib xyuas kev nce qib thiab kinetics ntawm kev sib sau ua ke.

Kev piav qhia txog kev sib cuam tshuam ntawm cov protein-protein: ANS tau siv los tshawb nrhiav kev sib cuam tshuam ntawm cov protein-protein. Thaum cov protein sib cuam tshuam, tej zaum yuav muaj kev hloov pauv hauv lawv qhov hydrophobicity, uas tuaj yeem saib xyuas tau siv ANS fluorescence.

Kev tshawb nrhiav thiab kev tsim tshuaj: ANS siv tau rau hauv kev tshuaj ntsuam cov neeg uas muaj peev xwm tsim tshuaj. Nws tuaj yeem pab soj ntsuam qhov kev sib txuas thiab kev sib cuam tshuam ntawm cov molecules me me nrog cov protein uas xav tau, uas ua rau cov kws tshawb fawb txheeb xyuas cov tshuaj uas tuaj yeem hloov kho kev ua haujlwm ntawm cov protein.

Khoom Ntim:

Cov Lus Qhia Ntxiv:

Kev sau ua ke	C16H13NO3S
Kev Ntsuas	99%
Qhov tsos	Hmoov ntsuab tsaus
CAS Nr.	82-76-8
Ntim khoom	Me me thiab ntau
Lub Neej Txee	2 xyoos
Kev Khaws Cia	Khaws cia rau qhov chaw txias thiab qhuav
Daim Ntawv Pov Thawj	ISO.