5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium ntsev CAS: 18656-96-7

Qhov cuam tshuam ntawm 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium ntsev (X-glucuronide) yog nws lub peev xwm los tsim cov xim xiav lossis hloov xim thaum muaj cov enzyme beta-D-glucuronidase. Qhov kev ua haujlwm enzymatic no tshwm sim thaum cov glycosidic bond ntawm pawg indole thiab cov glucuronic acid moiety raug cleaved los ntawm beta-D-glucuronidase.

Daim ntawv thov ntawm X-glucuronide yog feem ntau hauv kev sim molecular biology thiab enzymology. Nws feem ntau yog siv ua lub substrate los ntes cov haujlwm ntawm beta-D-glucuronidase. Qee qhov kev siv tshwj xeeb suav nrog:

Kev ntsuam xyuas cov noob caj noob ces ntawm tus neeg sau xov xwm: X-glucuronide feem ntau siv ua lub substrate hauv kev ntsuam xyuas cov noob caj noob ces ntawm tus neeg sau xov xwm qhov twg beta-D-glucuronidase siv ua lub enzyme ntawm tus neeg sau xov xwm. Los ntawm kev sib koom ua ke thaj chaw txhawb nqa lossis kev sau cov kab ke ntawm cov noob caj noob ces uas txaus siab rau cov noob caj noob ces beta-D-glucuronidase, cov kws tshawb fawb tuaj yeem ntsuas qhov kev qhia tawm lossis kev ua haujlwm ntawm cov noob caj noob ces los ntawm kev saib xyuas kev tsim cov dej xiav lossis kev hloov xim thaum ntxiv X-glucuronide.

Kev ntsuam xyuas kev hloov pauv ntawm cov kab mob thiab cov poov xab: X-glucuronide siv tau los ntes qhov kev hloov pauv zoo ntawm cov kab mob lossis poov xab nrog lub vector uas muaj cov noob beta-D-glucuronidase. Cov hlwb hloov pauv uas qhia cov enzyme tuaj yeem hydrolyze X-glucuronide, ua rau pom kev hloov pauv xim lossis nag lossis daus.

Kev siv xim histochemical: X-glucuronide siv rau hauv cov txheej txheem siv xim histochemical los pom qhov muaj thiab kev faib tawm ntawm beta-D-glucuronidase kev ua haujlwm hauv cov ntaub so ntswg lossis cov hlwb. Qhov kev siv xim no tso cai rau qhov chaw thiab kev txheeb xyuas cov hlwb uas qhia cov enzyme.