4-Methylumbelliferyl-beta-D-glucopyranoside CAS: 18997-57-4

Cov nyhuv ntawm 4-Methylumbelliferyl-beta-D-glucopyranoside (MUG) yog los ua lub substrate rau enzyme beta-glucosidase.Qhov no enzyme cleaves lub glucosidic daim ntawv cog lus ntawm MUG, uas ua rau tso tawm 4-Methylumbelliferone (4-MU).Kev thov ntawm MUG yog feem ntau nyob rau hauv lub teb ntawm microbiology, tshwj xeeb tshaj yog rau kev kuaj thiab txheeb xyuas ntawm beta-glucosidase-ua cov kab mob.MUG feem ntau yog siv los kuaj pom Escherichia coli (E. coli) hauv cov qauv dej thiab zaub mov.E. coli muaj cov enzyme beta-glucosidase, uas muaj peev xwm hydrolyze MUG thiab tsim ib lub teeb liab fluorescent nyob rau hauv lub xub ntiag ntawm ultraviolet (UV) lub teeb.Cov khoom fluorescent ntawm 4-MU tso cai rau kom yooj yim nrhiav thiab quantification.Thaum MUG substrate yog hydrolyzed, 4-MU generated emits xiav fluorescence, pab kom paub cov kab mob uas muaj beta-glucosidase kev ua.Txoj kev no feem ntau yog siv rau hauv kev saib xyuas ib puag ncig thiab kev soj ntsuam kev nyab xeeb ntawm zaub mov, vim nws muab txoj hauv kev sai thiab rhiab ntawm kev kuaj cov kab mob sib kis. Ntxiv rau nws daim ntawv thov hauv microbiology, MUG kuj tuaj yeem siv los kawm txog kev ua thiab inhibition ntawm beta-glucosidase hauv Biochemistry thiab molecular biology tshawb fawb.Nws cov fluorescence ua rau kev ntsuas ntawm enzyme kinetics thiab tuaj yeem siv los tshuaj xyuas cov muaj peev xwm inhibitors lossis activators ntawm beta-glucosidase kev ua haujlwm.Zuag qhia tag nrho, MUG yog ntau yam sib xyaw uas pom cov ntawv thov dav hauv thaj chaw ntawm microbiology, biochemistry, thiab molecular biology rau kev kuaj pom. ntawm beta-glucosidase kev ua haujlwm thiab kev txheeb xyuas cov kab mob uas tsim cov enzyme no.