X-GAL CAS: 7240-90-6 Chaw tsim tshuaj paus Nqe

Xim Hloov: X-Gal feem ntau tsis muaj xim tab sis, raws li hydrolysis los ntawm β-galactosidase, nws hloov xiav.Qhov kev hloov xim no tso cai rau pom qhov pom thiab ua kom muaj nuj nqis ntawm β-galactosidase kev ua haujlwm.

LacZ Gene Detection: X-Gal yog siv los txheeb xyuas cov hlwb lossis kev tsim cov caj ces uas qhia txog cov noob lacZ.LacZ feem ntau yog siv los ua tus neeg sau xov xwm gene hauv molecular biology los ntsuas cov noob qhia lossis kev kawm txhawb kev ua haujlwm.

Colony Screening: X-Gal feem ntau siv rau hauv kev tshuaj ntsuam kab mob kab mob.LacZ-expressing bacterial colonies tshwm xiav thaum loj hlob ntawm agar uas muaj X-Gal, ua kom yooj yim txheeb xyuas thiab xaiv cov lacZ-positive colonies.

Gene Fusion Analysis: X-Gal kuj tseem siv rau hauv cov kev sim gene fusion.Thaum lub hom phiaj gene txuas nrog lacZ gene, X-Gal staining tuaj yeem nthuav tawm cov qauv qhia ntawm fusion protein nyob rau hauv ib lub xovtooj ntawm tes lossis cov ntaub so ntswg.

Protein Localization: X-Gal staining tuaj yeem siv los tshawb xyuas subcellular protein localization.Los ntawm kev sib xyaw cov protein ntawm kev txaus siab rau cov noob lacZ, cov haujlwm β-galactosidase tuaj yeem qhia qhov twg cov protein nyob hauv ib lub cell.

X-Gal Analogues: Hloov cov ntaub ntawv ntawm X-Gal, xws li Bluo-Gal lossis Liab-Gal, tau tsim los tso cai rau lwm txoj kev tsim xim.Cov analogues no pab kom muaj kev sib txawv ntawm lacZ-positive thiab lacZ-negative hlwb lossis cov ntaub so ntswg siv cov xim sib txawv.