5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide yog ib qho chaw siv nyob rau hauv ntau yam kev tshawb fawb biochemical, tshwj xeeb tshaj yog rau kev kuaj pom thiab pom kev ua haujlwm ntawm enzyme.Nws yog ib qho substrate uas tuaj yeem hydrolyzed los ntawm cov enzymes tshwj xeeb, uas ua rau tso tawm cov khoom muaj xim lossis fluorescent.
Cov tshuaj no feem ntau siv rau hauv kev tshuaj ntsuam xyuas kom pom qhov muaj thiab kev ua haujlwm ntawm cov enzymes xws li beta-galactosidase thiab beta-glucuronidase.Cov enzymes no tshem tawm cov acetyl thiab glucosaminide pawg los ntawm cov substrate, ua rau tsim cov xim xiav lossis ntsuab chromophore.
Cov qauv tshwj xeeb ntawm 5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide tso cai rau kev tshawb nrhiav tau yooj yim thiab kom muaj nuj nqis ntawm kev ua enzyme.Nws siv nyob rau hauv ntau yam kev sim, suav nrog histochemistry, immunohistochemistry, thiab kev soj ntsuam ntawm tes, tau ua rau muaj kev nkag siab zoo ntawm kev ua haujlwm enzyme.