Muaj ntau tus phab ej uas tsis tau muaj npe nyob ib puag ncig peb, uas zoo li yog neeg ib txwm, tab sis qhov tseeb lawv pab ntau yam rau peb ntsiag to. Proteinase K yog "tus phab ej uas tsis tau muaj npe" hauv kev lag luam kuaj mob molecular, txawm hais tias piv rau "loj thiab muaj zog" hauv kev lag luam, proteinase K tsis tshua muaj txiaj ntsig uas peb tau saib tsis taus nws qhov tseem ceeb ntev lawm. Nrog rau kev sib kis ntawm tus kab mob tshiab, qhov kev thov rau proteinase K tau nce siab, thiab kev muab khoom hauv tsev thiab txawv teb chaws nyob deb ntawm kev noj, thiab txhua tus neeg mam li nco dheev tias proteinase K tseem ceeb heev.
Kev siv proteinase K yog dab tsi?
Proteinase K yog ib qho serine protease nrog cov proteolytic enzyme ua haujlwm thiab tuaj yeem tswj kev ua haujlwm hauv ntau qhov chaw ib puag ncig (pH (4-12.5), cov ntsev ntau, qhov kub siab ntawm 70 ° C, thiab lwm yam). Tsis tas li ntawd, cov haujlwm ntawm proteinase K tsis raug txwv los ntawm SDS, urea, EDTA, guanidine hydrochloride, guanidine isothiocyanate, thiab lwm yam, thiab qee qhov ntawm cov tshuaj ntxuav tes kuj tseem tuaj yeem txhim kho cov haujlwm ntawm proteinase K. Hauv kev kho mob (kab mob thiab microbial disinfection), zaub mov (nqaij mos), tawv (plaub hau mos), cawv (cawv clarification), amino acid npaj (plaub degraded), nucleic acid extraction, in situ hybridization, thiab lwm yam, proteinase K Muaj cov ntawv thov. Daim ntawv thov feem ntau yog nucleic acid extraction.
Proteinase K tuaj yeem enzymolyze txhua yam protein hauv cov qauv, suav nrog cov histones uas khi nruj rau nucleic acids, yog li cov nucleic acids tuaj yeem tso tawm ntawm cov qauv thiab tso tawm rau hauv cov extract, pab txhawb rau kauj ruam tom ntej ntawm kev rho tawm thiab kev ntxuav. Hauv kev kuaj pom cov kab mob nucleic acid, proteinase K yog ib qho ntawm cov khoom tseem ceeb hauv cov tshuaj kuaj kab mob. Proteinase K tuaj yeem tawg thiab ua kom cov protein tsho ntawm tus kab mob tsis ua haujlwm, uas muaj kev nyab xeeb dua thaum lub sijhawm thauj mus los thiab kuaj pom; ntxiv rau, proteinase K kuj tseem tuaj yeem rhuav tshem RNase tiv thaiv kev rhuav tshem ntawm kab mob RNA thiab pab txhawb kev kuaj pom nucleic acid.
Lub koob meej ntawm proteinase K thaum hmo ntuj
Txawm hais tias nyob rau hauv kev tshawb fawb txog kev tshawb fawb lossis hauv kev tshawb fawb IVD, kev rho tawm nucleic acid yog qhov kev sim yooj yim tshaj plaws, yog li proteinase K yeej ib txwm yog ib qho tseem ceeb heev. Txawm li cas los xij, yav dhau los, proteinase K tsis tshua paub zoo dua li nws lub luag haujlwm. Ib feem loj ntawm qhov no yog vim tias kev muab khoom thiab kev thov ntawm proteinase K ruaj khov heev. Ob peb tus neeg yuav xav tias kev muab khoom ntawm proteinase K yuav yog teeb meem.
Nrog rau kev sib kis ntawm tus kab mob tshiab crown, qhov kev thov rau kev kuaj nucleic acid tau nce siab. Txij li thaum kawg Lub Rau Hli 2020, Tuam Tshoj tau ua tiav yuav luag 90 lab qhov kev kuaj crown tshiab, thiab tus lej no tseem ceeb dua rau thoob ntiaj teb. Hauv kev sim rho tawm nucleic acid, qhov ua haujlwm concentration ntawm proteinase K yog li 50-200 μg / mL. Feem ntau, nws yuav siv li 100 μg ntawm proteinase K los rho tawm cov qauv ntawm nucleic acid. Hauv kev siv tiag tiag, txhawm rau kom ua kom zoo dua ntawm kev rho tawm nucleic acid, feem ntau Proteinase K yuav raug siv ntau dua. Kev kuaj pom nucleic acid ntawm tus kab mob coronavirus tshiab tau coj ntau qhov kev thov proteinase K. Qhov sib npaug ntawm kev muab khoom thiab kev thov ntawm proteinase K tau tawg sai sai, thiab proteinase K tau dhau los ua cov khoom siv tiv thaiv kev sib kis tseem ceeb hauv ib hmos.
Qhov nyuaj hauv kev tsim cov proteinase K
Txawm hais tias nrog kev loj hlob ntawm tus kab mob sib kis, qhov tseem ceeb ntawm proteinase K tau raug suav tias yog qhov tseem ceeb los ntawm tib neeg, nws yog qhov txaj muag uas vim yog qhov qis qis ntawm proteinase K, ob peb lub tuam txhab hauv tsev tau koom nrog hauv kev tsim cov proteinase K. Thaum tib neeg xav tsim kom muaj proteinase K ntau lawm Thaum lub sijhawm tsim khoom, nws tau pom tias proteinase K yog cov protein tshwj xeeb heev. Nws yog qhov nyuaj heev rau kev nthuav dav lub peev xwm tsim khoom ntawm proteinase K hauv lub sijhawm luv luv.
Kev tsim cov proteinase K ntau ntau ntsib cov teeb meem hauv qab no:
1. Kev qhia tawm qis
Proteinase K tuaj yeem ua rau feem ntau cov protein puas tsuaj tsis yog tshwj xeeb thiab ua rau muaj kev lom loj rau lub cell uas tus tswv tsev ua haujlwm. Yog li ntawd, qib kev ua haujlwm ntawm proteinase K feem ntau qis heev. Kev tshuaj xyuas cov kab ke ua haujlwm thiab cov kab mob uas ua rau muaj proteinase K ntau heev feem ntau xav tau lub voj voog ntev dua.
2. Cov seem ntawm cov xim thiab cov kua qaub nucleic
Kev fermentation loj ua rau muaj ntau cov xim thiab cov nucleic acid residues. Nws nyuaj rau tshem tawm cov impurities no nrog cov txheej txheem ntxuav yooj yim, thiab kev ntxuav nyuaj ua rau tus nqi nce thiab txo qhov nrawm rov qab.
3. Kev Tsis Ruaj Khov
Proteinase K tsis ruaj khov txaus, nws tuaj yeem enzymolyze nws tus kheej, thiab nws nyuaj rau khaws cia ruaj khov ntawm 37 ° C ntev yam tsis muaj tus neeg sawv cev tiv thaiv.
4. Yooj yim rau precipitate
Thaum npaj cov hmoov proteinase K khov-qhuav, txhawm rau kom ntseeg tau tias cov khoom khov ntawm proteinase K hauv cov hmoov khov-qhuav loj, nws yog qhov tsim nyog los ntxiv cov neeg sawv cev tiv thaiv khov-qhuav ntawm qhov concentration siab, tab sis thaum qhov concentration ntawm proteinase K ncav cuag 20mg / mL thiab siab dua, nws yooj yim Qhov kev sib sau ua ke tsim cov precipitate, uas coj teeb meem loj rau kev khov-qhuav ntawm proteinase K nrog cov khoom khov siab.
5. Kev nqis peev loj
Proteinase K muaj cov haujlwm protease muaj zog thiab tuaj yeem hydrolyze lwm cov proteases hauv chav kuaj mob. Yog li ntawd, proteinase K xav tau cov chaw tsim khoom tshwj xeeb, cov khoom siv, thiab cov neeg ua haujlwm rau kev tshawb fawb thiab kev txhim kho thiab kev tsim khoom.
XD BIOCHEM cov tshuaj proteinase K
XD BIOCHEM muaj lub platform qhia txog cov protein thiab kev ntxuav kom huv si, thiab muaj kev paub nplua nuj hauv kev qhia thiab kev ntxuav cov protein recombinant thiab kev ua kom zoo dua ntawm cov txheej txheem tsim khoom. Los ntawm kev tsim sai ntawm pab pawg tshawb fawb thiab kev txhim kho, cov txheej txheem tsim khoom loj ntawm proteinase K tau kov yeej. Cov khoom tsim tawm txhua hli ntawm cov hmoov khov-qhuav yog ntau dua 30 KG. Cov khoom muaj kev ua haujlwm ruaj khov, kev ua haujlwm tshwj xeeb enzyme siab, thiab tsis muaj cov khoom seem cytochrome thiab nucleic acid. Txais tos tiv tauj XD BIOCHEM Tau txais pob khoom sim (E-mail:sales@xdbiochem.comXov tooj: +86 513 81163739).
XD BIOCHEM cov kev daws teeb meem suav nrog
Siv ntau daim ntawv theej plasmid kev sib koom ua ke, cov kab mob siab qhia tawm nrog qib kev qhia tawm ntawm 8g / L raug xaiv, uas kov yeej qhov teeb meem ntawm qib kev qhia tawm qis ntawm proteinase K.
Los ntawm kev tsim kom muaj ntau kauj ruam purification, tus tswv tsev cytochrome thiab nucleic acid residues ntawm proteinase K tau raug tshem tawm hauv qab tus nqi txheem.
Los ntawm kev tshuaj ntsuam xyuas cov qauv tiv thaiv buffer siab, ib lub buffer uas tuaj yeem khaws cov proteinase K ntawm 37 ° C tau raug xaiv.
Cov tshuaj ntsuam xyuas kov yeej qhov teeb meem uas proteinase K yooj yim sib sau ua ke thiab ua rau muaj kev kub ntxhov ntau, thiab tso lub hauv paus rau proteinase K cov khoom khov-qhuav uas muaj cov khoom khov kho.
XD BIOCHEM proteinase K qauv
XD BIOCHEM proteinase K kev ntsuas ruaj khov: yuav tsis muaj kev hloov pauv tseem ceeb hauv kev ua haujlwm tom qab 80 hnub ntawm chav tsev kub
XD BIOCHEM proteinase K kev ruaj khov xeem: yuav tsis muaj kev hloov pauv tseem ceeb hauv kev ua haujlwm tom qab 80 hnub ntawm chav tsev kub.
Kev sib piv ntawm cov txiaj ntsig ntawm kev rho tawm nucleic acid ntawm XD BIOCHEM proteinase K thiab cov khoom sib tw. Hauv cov txheej txheem ntawm kev rho tawm nucleic acid, XD BIOCHEM thiab kev sib tw proteinase K raug siv raws li. Kev ua haujlwm rho tawm ntawm XD BIOCHEM proteinase K siab dua thiab tus nqi Ct ntawm lub hom phiaj gene qis dua.
Lub sijhawm tshaj tawm: Lub Kaum Ob Hlis-31-2021