Muaj ntau tus neeg tsis paub zoo nyob ib puag ncig peb, uas zoo li zoo li qub, tab sis qhov tseeb lawv ua ntsiag to pab ntau rau peb.Proteinase K yog "unsung hero" nyob rau hauv kev lag luam molecular diagnostics, txawm hais tias piv nrog "loj thiab muaj zog" hauv kev lag luam, proteinase K yog qhov tseem ceeb uas peb tau saib ntev ntev nws qhov tseem ceeb.Nrog rau qhov tshwm sim ntawm tus kab mob tshiab, qhov kev thov rau proteinase K tau nce siab, thiab cov khoom siv hauv tsev thiab txawv teb chaws yog nyob deb ntawm kev noj, thiab txhua tus neeg mam li nco dheev pom tias proteinase K tseem ceeb heev.
Kev siv cov proteinase K yog dab tsi?
Proteinase K yog ib tug serine protease nrog proteolytic enzyme kev ua si thiab muaj peev xwm tswj tau kev ua ub no nyob rau hauv ib tug ntau yam ntawm ib puag ncig (pH (4-12.5), high- ntsev tsis, kub ntawm 70 ° C, thiab lwm yam.).Tsis tas li ntawd, kev ua haujlwm ntawm proteinase K tsis cuam tshuam los ntawm SDS, urea, EDTA, guanidine hydrochloride, guanidine isothiocyanate, thiab lwm yam., thiab qee qhov ntawm cov tshuaj ntxuav tes kuj tuaj yeem txhim kho kev ua haujlwm ntawm proteinase K. Hauv kev kho mob (tus kab mob thiab tshuaj tua kab mob microbial. ), khoom noj khoom haus (nqaij tenderization), tawv (cov plaub hau softening), winemaking (cawv clarification), amino acid npaj (degraded feathers), nucleic acid extraction, nyob rau hauv situ hybridization, thiab lwm yam, proteinase K muaj kev siv.Daim ntawv thov feem ntau siv yog nucleic acid extraction.
Proteinase K tuaj yeem enzymolyze txhua yam ntawm cov proteins hauv cov qauv, suav nrog cov histones uas nruj nreem khi rau nucleic acids, kom cov nucleic acids tuaj yeem tso tawm los ntawm cov qauv thiab tso tawm rau hauv cov extract, yooj yim rau cov kauj ruam tom ntej ntawm kev rho tawm thiab purification.Hauv kev kuaj pom tus kab mob nucleic acid, proteinase K yog ib qho tseem ceeb ntawm cov tshuaj tua kab mob.Proteinase K tuaj yeem tawg thiab ua tsis taus lub tsho tiv no ntawm tus kab mob, uas muaj kev nyab xeeb dua thaum lub sijhawm thauj mus los thiab kuaj pom;Tsis tas li ntawd, proteinase K kuj tseem tuaj yeem txo qis RNase tiv thaiv kev degradation ntawm tus kab mob RNA thiab pab txhawb kev tshawb nrhiav nucleic acid.
Lub koob meej hmo ntuj ntawm proteinase K
Txawm hais tias nyob rau hauv kev tshawb fawb ntawm kev tshawb fawb los yog hauv thaj tsam ntawm IVD, nucleic acid extraction yog qhov kev sim yooj yim tshaj plaws, yog li proteinase K yeej ib txwm muaj qhov tseem ceeb heev.Txawm li cas los xij, yav dhau los, proteinase K tsis tshua paub zoo dua li nws lub luag haujlwm.Ib feem loj ntawm qhov no yog vim qhov khoom thiab kev xav tau kev sib raug zoo ntawm proteinase K yog ruaj khov heev.Tsawg tus neeg yuav xav tias kev muab cov proteinase K yuav yog qhov teeb meem.
Nrog rau qhov tshwm sim ntawm tus kabmob tshiab, qhov kev thov rau kev kuaj nucleic acid tau nce ntxiv.Txog rau thaum lub Rau Hli 2020, Tuam Tshoj tau ua tiav ze li ntawm 90 lab qhov kev sim tshiab tshiab, thiab tus lej no tseem ceeb dua rau thoob ntiaj teb.Hauv kev sim nucleic acid extraction, kev ua haujlwm ntawm cov proteinase K yog li 50-200 μg / mL.Feem ntau, nws yuav siv li 100 μg ntawm cov proteinase K kom rho tawm cov qauv ntawm nucleic acid.Hauv kev siv tiag tiag, txhawm rau txhawm rau ua kom muaj txiaj ntsig ntawm nucleic acid extraction, feem ntau Proteinase K yuav siv tau ntau ntxiv.Nucleic acid kuaj pom tus kabmob tshiab no tau coj ntau cov proteinase K xav tau.Cov khoom qub thiab kev xav tau ntawm cov proteinase K tau tawg sai sai, thiab proteinase K tau dhau los ua cov khoom tseem ceeb tiv thaiv kab mob thaum hmo ntuj.
Teeb meem hauv kev tsim cov proteinase K
Txawm hais tias nrog rau kev loj hlob ntawm kev sib kis, qhov tseem ceeb ntawm cov proteinase K tau txais txiaj ntsig los ntawm tib neeg, nws yog qhov txaj muag vim tias qhov tseem ceeb ntawm cov proteinase K ntau dhau, ob peb lub tuam txhab hauv tsev tau koom nrog hauv kev tsim cov proteinase K. Thaum tib neeg xav tsim cov proteinase K ntau lawm Thaum lub sijhawm tsim khoom, nws tau tshawb pom tias proteinase K yog cov protein tshwj xeeb heev.Nws yog qhov nyuaj heev los nthuav tawm cov peev txheej ntawm cov proteinase K hauv lub sijhawm luv luv.
Qhov loj-scale zus tau tej cov proteinase K ntsib cov teeb meem nram qab no
1. Kev qhia qis
Proteinase K tuaj yeem tsis yog qhov tshwj xeeb degrade cov protein ntau thiab ua rau muaj kev phom sij loj rau kev qhia tus tswv ntawm tes.Yog li ntawd, qhov kev qhia ntawm proteinase K yog feem ntau qis heev.Kev tshuaj ntsuam ntawm cov kab lus qhia thiab hom kab mob uas nthuav tawm cov proteinase K feem ntau yuav tsum tau muaj lub voj voog ntev dua.
2. Cov xim ntawm cov pigments thiab nucleic acids
Loj-loj fermentation qhia ntau cov xim thiab cov tswv tsev nucleic acid residues.Nws yog ib qho nyuaj rau tshem tawm cov impurities nrog ib tug yooj yim purification txheej txheem, thiab complex purification tsub kom tus nqi thiab txo tus nqi rov qab.
3. Tsis ruaj khov
Proteinase K tsis ruaj khov txaus, nws muaj peev xwm enzymolyze nws tus kheej, thiab nws yog ib qho nyuaj rau khaws cia ruaj khov ntawm 37 ° C rau lub sij hawm ntev yam tsis muaj tus neeg sawv cev tiv thaiv.
4. Yooj yim los nag
Thaum npaj cov hmoov khov-qhuav ntawm cov proteinase K, txhawm rau kom ntseeg tau tias cov ntsiab lus ntawm cov proteinase K hauv cov hmoov khov-qhuav yog loj, nws yog ib qho tsim nyog yuav tsum tau ntxiv cov tshuaj tiv thaiv khov-qhuav ntawm qhov siab, tab sis thaum lub caij ntuj no. concentration ntawm proteinase K nce mus txog 20mg / mL thiab siab dua, nws yog ib qho yooj yim Lub aggregation ua ib tug precipitate, uas coj teeb meem loj rau lub freeze-drying ntawm proteinase K nrog ib tug siab cov ntsiab lus.
5. Kev nqis peev loj
Proteinase K muaj zog tiv thaiv kev ua haujlwm thiab tuaj yeem ua rau hydrolyze lwm cov proteases hauv chav kuaj.Yog li ntawd, proteinase K xav tau cov chaw tsim khoom tshwj xeeb, khoom siv, thiab cov neeg ua haujlwm rau kev tshawb fawb thiab kev tsim kho thiab tsim khoom.
XD BIOCHEM's proteinase K daws
XD BIOCHEM muaj cov lus qhia paub protein ntau thiab purification platform, thiab muaj kev paub nplua nuj nyob rau hauv kev qhia thiab purification ntawm recombinant proteins thiab optimization ntawm ntau lawm txheej txheem.Los ntawm kev tsim sai ntawm pab pawg tshawb fawb thiab kev tsim kho, cov txheej txheem loj ntawm cov proteinase K tau kov yeej.Cov zis txhua hli ntawm cov hmoov qhuav qhuav yog ntau tshaj 30 KG.Cov khoom lag luam muaj kev ua haujlwm ruaj khov, siab enzyme tshwj xeeb ua haujlwm, thiab tsis muaj tus tswv tsev cytochrome thiab nucleic acid residues.Zoo siab txais tos hu rau XD BIOCHEM Tau txais pob khoom sim (E-mail:sales@xdbiochem.comXov tooj: +86 513 81163739).
XD BIOCHEM cov kev daws teeb meem suav nrog
Siv ntau daim ntawv theej plasmid kev sib koom ua ke, cov kab lus hais tawm siab nrog kev qhia theem ntawm 8g / L raug xaiv, uas kov yeej qhov teeb meem ntawm qib qis ntawm proteinase K.
Los ntawm kev tsim cov txheej txheem purification ntau kauj ruam, tus tswv tsev cytochrome thiab nucleic acid residues ntawm proteinase K tau ntse tshem tawm hauv qab tus nqi qauv.
Los ntawm kev tshuaj ntsuam xyuas ntawm kev tiv thaiv tsis zoo, ib qho tsis tuaj yeem khaws cov proteinase K ntawm 37 ° C.
Kev tshuaj ntsuam buffers kov yeej qhov teeb meem uas proteinase K yog ib qho yooj yim rau aggregate thiab precipitate ntawm high concentrations, thiab nteg lub hauv paus rau proteinase K cov khoom siab cov ntsiab lus khov-drying.
XD BIOCHEM proteinase K qauv
XD BIOCHEM proteinase K stability test: yuav tsis muaj qhov hloov pauv tseem ceeb hauv kev ua haujlwm tom qab 80 d ntawm chav tsev kub
XD BIOCHEM proteinase K stability test: yuav tsis muaj qhov hloov pauv tseem ceeb hauv kev ua haujlwm tom qab 80 d ntawm chav tsev kub.
Kev sib piv ntawm nucleic acid extraction nyhuv ntawm XD BIOCHEM proteinase K thiab cov khoom sib tw.Hauv cov txheej txheem ntawm nucleic acid extraction, XD BIOCHEM thiab kev sib tw proteinase K yog siv raws li.Lub extraction efficiency ntawm XD BIOCHEM proteinase K yog siab dua thiab Ct tus nqi ntawm lub hom phiaj gene yog qis dua.
Post lub sij hawm: Dec-31-2021