NBD chloride CAS: 10199-89-0

Kev cim covalent: NBD chloride tuaj yeem tsim cov kev sib txuas covalent nrog cov pab pawg nucleophilic hauv biomolecules, xws li cov amines thawj, thiols, thiab cov pab pawg hydroxyl. Qhov no tso cai rau kev cim tshwj xeeb ntawm cov biomolecules no rau kev kuaj pom thiab thaij duab.

Kev txhim kho fluorescence: Thaum NBD chloride cuam tshuam nrog nucleophiles, nws tsim cov khoom fluorescent NBD-labeled. Cov khoom lag luam no qhia txog kev tso tawm fluorescence muaj zog, ua rau nws muaj txiaj ntsig zoo rau kev pom thiab taug qab cov molecules uas tau cim.

Kev rhiab heev thiab qhov tshwj xeeb: Cov khoom lag luam uas muaj daim ntawv lo NBD muaj kev rhiab heev, ua rau pom tau cov molecule me me uas muaj daim ntawv lo. Ntxiv mus, qhov kev sib txuas ntawm NBD chloride thiab nucleophiles muab kev tshwj xeeb, vim nws ua kom muaj kev sib txuas ruaj khov thiab tshwj xeeb ntawm cov xim rau lub hom phiaj biomolecule.

Kev sau npe rau cov biomolecule: NBD chloride siv tau los sau npe rau ntau yam biomolecules, suav nrog cov protein, peptides, nucleic acids, thiab lwm yam me me. Qhov no ua rau cov kws tshawb fawb tshawb nrhiav qhov chaw, kev hloov pauv, thiab kev sib cuam tshuam ntawm cov biomolecules no hauv cov kab ke ntawm lub cev.

Kev Siv Lub Tshuab Microscopy Uas Muaj Teeb Ci NBD: Cov biomolecules uas muaj daim ntawv lo NBD tuaj yeem pom tau los ntawm kev siv lub tshuab microscopy uas muaj teeb ci fluorescence. Cov txheej txheem no tso cai rau kev soj ntsuam cov molecules uas muaj daim ntawv lo ntawm theem cellular lossis subcellular, muab cov ntaub ntawv tseem ceeb txog lawv qhov kev faib tawm thiab tus cwj pwm.

Kev ntsuas cov kua dej ntws: Cov tshuaj biomolecules uas muaj daim ntawv lo NBD tuaj yeem tshuaj xyuas thiab ntsuas tau los ntawm kev siv cov kua dej ntws. Cov txheej txheem no tso cai rau kev tshuaj xyuas cov hlwb lossis cov khoom me me uas muaj daim ntawv lo, muab cov ntaub ntawv hais txog cov qib kev qhia tawm lossis cov khoom ntawm cov tshuaj biomolecules uas muaj daim ntawv lo.

Kev sau npe thiab taug qab cov protein: NBD chloride siv tau los sau npe cov amino acid residues lossis cov pab pawg ua haujlwm hauv cov protein. Qhov no ua rau cov kws tshawb fawb kawm txog cov qauv protein, kev sib sau ua ke, kev hloov pauv, thiab kev sib cuam tshuam hauv lub sijhawm tiag tiag.