N-Ethylmaleimide CAS: 128-53-0 Tus nqi tsim khoom

Kev Hloov Kho Protein: NEM hloov kho cov cysteine ​​residues hauv cov protein los ntawm kev tsim cov adduct covalent. Qhov kev hloov kho no tuaj yeem cuam tshuam rau kev ua haujlwm ntawm cov protein, cov qauv, thiab kev sib cuam tshuam. NEM qhov kev ua haujlwm nrog cov pab pawg sulfhydryl ua rau nws yog ib qho cuab yeej muaj txiaj ntsig zoo rau kev kawm txog kev ua haujlwm ntawm cov protein thiab cov txheej txheem uas nyob ntawm thiol.

Kev Txwv Cov Enzymes: Ntau cov enzymes vam khom cov pab pawg thiol rau lawv cov haujlwm catalytic. Los ntawm kev cuam tshuam nrog cov pab pawg thiol, NEM tuaj yeem xaiv cov enzymes tshwj xeeb. Qhov no tau siv dav los kawm txog enzyme kinetics, nkag siab txog cov txheej txheem enzymatic, thiab tshuaj xyuas cov peev xwm enzyme inhibitors.

Kev Tshawb Fawb Txog Kev Sib Cuam Tshuam ntawm Cov Protein-Protein: NEM feem ntau siv los tshawb nrhiav kev sib cuam tshuam ntawm cov protein-protein. Los ntawm kev xaiv thaiv cov cysteine ​​​​​​residues uas ua rau muaj kev cuam tshuam, NEM tiv thaiv kev tsim cov disulfide bonds ntawm cov protein sib cuam tshuam, yog li cuam tshuam lawv kev sib cuam tshuam. Qhov no tso cai rau cov kws tshawb fawb kawm txog lub luag haujlwm ntawm cov cysteine ​​​​​​tshwj xeeb hauv kev sib cuam tshuam ntawm cov protein-protein.

Kev Tshawb Fawb Txog Kev Tswj Xyuas Redox: NEM siv rau hauv kev tshawb fawb txog cov txheej txheem tswj hwm redox hauv cov protein. Los ntawm kev hloov kho cov cysteine ​​​​​​uas muaj zog, NEM tuaj yeem ua kom cov protein hloov pauv tau ruaj khov thiab tiv thaiv kev oxidation lossis txo qis ntawm cov cysteine ​​​​​​uas tsis xav tau. Qhov no pab nkag siab txog lub luag haujlwm ntawm redox signaling thiab redox-sensitive regulatory pathways.

Kev Tshawb Fawb Txog Kev Sib Txuas: NEM siv tau ua tus neeg sawv cev sib txuas los tswj cov protein complexes lossis kev sib cuam tshuam ntawm cov protein-protein. Los ntawm kev hloov kho cov cysteine ​​​​​​cov residues, NEM tuaj yeem tsim cov kev sib txuas ntawm cov protein sib cuam tshuam, uas ua rau cov kws tshawb fawb tshawb nrhiav cov protein complexes thiab lawv cov qauv. Cov txheej txheem no muaj txiaj ntsig zoo hauv kev tshawb fawb txog biology thiab proteomics.

Kev Tshawb Pom Tshuaj: Vim nws muaj peev xwm xaiv tau kev sib cuam tshuam nrog cov cysteine ​​​​​​cov residues, NEM tau siv rau hauv kev tshawb fawb tshawb pom tshuaj. Nws tuaj yeem siv los txheeb xyuas thiab piav qhia txog cov hom phiaj tshuaj lossis kawm txog qhov tshwj xeeb ntawm cov tshuaj sib tw rau cov protein nplua nuj cysteine.