HEPES CAS: 7365-45-9

HEPES ua lub luag haujlwm tseem ceeb hauv kev tswj hwm ib puag ncig pH ruaj khov hauv cov kab lis kev cai cell, ua kom muaj kev muaj sia nyob thiab kev loj hlob ntawm cov cell los ntawm kev tiv thaiv kev hloov pauv hauv pH. Lub peev xwm buffering ntawm HEPES no yog qhov tseem ceeb tshwj xeeb hauv kev tswj hwm cov xwm txheej physiological rau kev tshawb fawb raws li cell thiab kev kuaj tshuaj, ua rau muaj kev ntseeg siab dua thiab rov ua dua. Hauv molecular biology thiab biochemistry, HEPES feem ntau siv rau hauv enzyme assays los tswj pH-sensitive enzymatic reactions zoo. Nws lub peev xwm los tswj hwm pH tas li hauv qhov ntau thiab tsawg (feem ntau 7.2-7.6) yog qhov tseem ceeb rau kev ua kom raug thiab ua haujlwm ntawm cov txheej txheem biochemical, xws li protein purification, nucleic acid analysis, thiab metabolic studies. HEPES kuj tseem siv rau hauv electrophoresis daim ntawv thov, suav nrog agarose gel electrophoresis thiab polyacrylamide gel electrophoresis, qhov twg nws pab tsim ib puag ncig zoo tshaj plaws rau DNA thiab protein sib cais. Nws cov khoom buffering pab txhawb rau cov bands ntse thiab zoo-txhais, txhim kho qhov kev daws teeb meem thiab qhov tseeb ntawm cov txiaj ntsig sim. Ntxiv mus, HEPES pom kev siv dav hauv kev lag luam tshuaj rau kev tsim cov khoom siv biological, tshuaj tiv thaiv, thiab cov tshuaj kuaj mob. Nws qhov sib xws nrog cov biomolecules rhiab heev thiab qis cytotoxicity ua rau nws yog qhov kev xaiv zoo tshaj plaws rau kev tswj hwm qhov ruaj khov thiab kev ua haujlwm ntawm biopharmaceuticals thaum lub sijhawm tsim khoom thiab khaws cia. Zuag qhia tag nrho, HEPES ua haujlwm ua tus neeg sawv cev buffering ntau yam thiab tsis tuaj yeem tsis tau nrog ntau yam kev siv hauv cell biology, kev tshawb fawb molecular, biochemistry, thiab kev tsim tshuaj, ua lub luag haujlwm tseem ceeb hauv kev ua kom muaj kev vam meej thiab kev ntseeg siab ntawm ntau yam kev sim tshuaj lom neeg thiab biochemical.