Geneticin disulfate (G418 sulfate) CAS: 108321-42-2

Hauv chaw kuaj mob, geneticin disulfate (G418 sulfate) feem ntau siv rau hauv kev sim kab lis kev cai los xaiv thiab tswj cov kab mob cell uas muaj cov vectors qhia tawm nrog cov noob tiv thaiv, xws li neomycin phosphotransferase. Cov cell no tuaj yeem muaj sia nyob hauv qhov muaj geneticin disulfate vim yog kev qhia tawm ntawm cov noob tiv thaiv, tso cai rau cov kws tshawb fawb cais tawm thiab kawm txog cov pej xeem cell hloov kho caj ces. Cov kws tshawb fawb siv geneticin disulfate hauv ntau yam kev siv, suav nrog kev tsim cov kab mob cell ruaj khov, cov txheej txheem kho noob xws li CRISPR-Cas9, thiab kev tsim cov protein recombinant. Los ntawm kev koom ua ke geneticin disulfate rau hauv cov kab lis kev cai, cov pej xeem cell tshwj xeeb tuaj yeem nplua nuj lossis cais tawm raws li lawv lub peev xwm los muaj sia nyob xaiv cov tshuaj tua kab mob. Kev ua kom zoo tshaj plaws ntawm kev sib xyaw thiab lub sijhawm ntawm kev kho mob geneticin disulfate yog qhov tseem ceeb kom ua tiav kev xaiv siab thaum txo qis cytotoxicity thiab cov teebmeem tawm ntawm cov cell tsis transfected. Kev saib xyuas zoo ntawm kev muaj sia nyob ntawm cell, cov yam ntxwv kev loj hlob, thiab cov qauv qhia noob pab ua kom muaj txiaj ntsig zoo thaum siv geneticin disulfate hauv kev tshawb fawb txog kab lis kev cai. Cov kws tshawb nrhiav yuav tsum ua raws li cov txheej txheem uas tau tsim tsa rau kev siv geneticin disulfate, suav nrog kev noj tshuaj, kev tuav, thiab kev pov tseg kom raug, kom tswj tau cov qauv kev nyab xeeb hauv chaw kuaj mob thiab txhawb nqa cov txiaj ntsig kev tshawb fawb tseeb. Kev koom tes ntawm cov kws tshawb fawb, cov neeg ua haujlwm hauv chaw kuaj mob, thiab cov tub ceev xwm tswj hwm txhawb nqa kev siv geneticin disulfate uas muaj lub luag haujlwm thiab zoo hauv kev tshawb fawb txog molecular biology, txhawb peb txoj kev nkag siab txog cov txheej txheem ntawm tes thiab cov txheej txheem kev tswj hwm genetic.