FLUORESCEIN MONO-BETA-D- GALACTOPYRANOSIDE CAS: 102286-67-9

Fluorescein mono-beta-D-galactopyranoside (FMG) yog ib qho molecule uas feem ntau siv rau hauv kev tshawb fawb txog tsiaj txhu ua ib qho substrate los ntes qhov muaj thiab kev ua haujlwm ntawm beta-galactosidase enzyme. FMG yog ib qho derivative ntawm qab zib lactose thiab yog conjugated nrog ib qho molecule fluorescein.

Qhov cuam tshuam tseem ceeb ntawm FMG yog tias nws raug hydrolyzed tshwj xeeb los ntawm beta-galactosidase, ib qho enzyme uas rhuav cov lactose ua galactose thiab glucose. Qhov enzymatic hydrolysis ntawm FMG no ua rau muaj kev tso tawm ntawm fluorescein, uas tso tawm lub teeb liab fluorescence muaj zog.

Daim ntawv thov tseem ceeb ntawm FMG yog nyob rau hauv kev ntes thiab ntsuas beta-galactosidase kev ua ub no hauv ntau yam qauv. Cov enzyme no pom muaj nyob rau hauv ntau yam kab mob, suav nrog cov kab mob thiab cov tsiaj txhu, thiab nws cov haujlwm tuaj yeem qhia txog ntau yam txheej txheem cellular thiab cov txheej txheem metabolic.

Los ntawm kev siv FMG ua ib qho substrate, beta-galactosidase kev ua ub no tuaj yeem ntsuas tau los ntawm kev saib xyuas qhov fluorescence uas tso tawm los ntawm cov fluorescein uas tau tso tawm. Qhov kev ntsuas no tuaj yeem ua tiav hauv ntau yam kev teeb tsa kev sim, suav nrog kev sim hauv vitro thiab kev tshawb fawb txog cov hlwb muaj sia.

Ntxiv mus, FMG tuaj yeem siv ua ib qho cuab yeej los kawm txog kev faib tawm thiab qhov chaw ntawm beta-galactosidase hauv cov hlwb. Los ntawm kev siv cov txheej txheem fluorescent microscopy, cov kws tshawb fawb tuaj yeem pom qhov fluorescence uas FMG tso tawm thaum hydrolysis, uas tso cai rau lawv taug qab qhov chaw thiab lub sijhawm ua haujlwm ntawm beta-galactosidase.