FLUORESCEIN MONO-BETA-D- GALACTOPYRANOSIDE CAS TSIS TAU: 102286-67-9

Fluorescein mono-beta-D-galactopyranoside (FMG) yog ib qho molecule uas feem ntau siv hauv kev tshawb fawb lom neeg ua lub substrate los xyuas qhov muaj thiab kev ua ntawm beta-galactosidase enzyme.FMG yog ib qho derivative ntawm qab zib lactose thiab yog conjugated nrog fluorescein molecule.

Cov txiaj ntsig tseem ceeb ntawm FMG yog qhov tshwj xeeb hydrolyzed los ntawm beta-galactosidase, ib qho enzyme uas zom lactose rau hauv galactose thiab qabzib.Qhov enzymatic hydrolysis ntawm FMG ua rau kev tso tawm ntawm fluorescein, uas tso tawm cov teeb liab muaj zog fluorescence.

Thawj daim ntawv thov ntawm FMG yog nyob rau hauv kev kuaj thiab ntsuas beta-galactosidase kev ua hauv ntau yam qauv.Cov enzyme no muaj nyob rau hauv ntau yam kab mob, nrog rau cov kab mob thiab mammalian hlwb, thiab nws cov dej num yuav qhia tau hais tias ntawm ntau yam cellular txheej txheem thiab metabolic txoj kev.

Los ntawm kev siv FMG ua lub substrate, beta-galactosidase kev ua haujlwm tuaj yeem ntsuas los ntawm kev saib xyuas cov fluorescence tawm los ntawm kev tso tawm fluorescein.Qhov kev ntsuas no tuaj yeem ua tiav hauv ntau yam kev sim teeb tsa, suav nrog hauv vitro kev soj ntsuam thiab kev tshawb fawb ntawm tes nyob.

Tsis tas li ntawd, FMG tuaj yeem siv los ua ib qho cuab yeej los kawm txog kev faib khoom thiab chaw nyob ntawm beta-galactosidase hauv cov hlwb.Los ntawm kev siv cov txheej txheem fluorescent microscopy, cov kws tshawb fawb tuaj yeem pom cov fluorescence tso tawm los ntawm FMG raws li hydrolysis, tso cai rau lawv taug qab cov kev ua ub no thiab lub cev ntawm beta-galactosidase.