Fluorescein Isothiocyante CAS: 3326-32-7

Cov yam ntxwv ntawm fluorescence: FITC muaj cov txiaj ntsig quantum siab thiab tso tawm cov fluorescence ntsuab ci ntsa iab thaum muaj kev txhawb nqa ntawm ib ncig ntawm 488 nm. Nws cov yam ntxwv fluorescence ua rau nws tsim nyog rau kev kuaj pom siv ntau yam txheej txheem duab, xws li fluorescence microscopy, flow cytometry, thiab fluorescence spectroscopy.

Kev khi covalent: FITC muaj ib pawg isothiocyanate uas tso cai rau nws khi cov amino pawg uas muaj nyob rau hauv cov protein, cov tshuaj tiv thaiv kab mob, thiab lwm yam biomolecules. Qhov kev khi covalent no ua kom ruaj khov thiab tshwj xeeb daim ntawv lo ntawm cov molecules lub hom phiaj, ua rau lawv nrhiav tau thiab pom tau.

Immunofluorescence: Cov tshuaj tiv thaiv kab mob uas muaj daim ntawv lo FITC feem ntau siv rau hauv kev ntsuam xyuas immunofluorescence los nrhiav cov antigens lossis cov protein tshwj xeeb hauv cov cell thiab cov nqaij. Cov xim ntsuab ci ntsa iab uas FITC tso tawm ua rau cov kws tshawb fawb pom thiab pom qhov kev faib tawm ntawm cov molecule hauv cov qauv tshuaj lom neeg.

Kev ntsuas cov hlwb ntws: Cov tshuaj tiv thaiv kab mob uas muaj daim ntawv lo FITC lossis lwm cov tshuaj fluorochrome-conjugated biomolecules yog siv dav hauv kev ntsuas cov hlwb ntws los tshuaj xyuas thiab cais cov hlwb raws li lawv cov cim qhia saum npoo lossis cov khoom hauv lub hlwb. FITC sib xws nrog 488 nm laser uas feem ntau siv hauv cov cuab yeej ntsuas cov hlwb ntws.

Kev tshawb nrhiav txog kev sib cuam tshuam ntawm cov protein: FITC siv tau rau hauv kev tshawb nrhiav kev sib cuam tshuam ntawm cov protein thiab cov protein los ntsuam xyuas qhov kev sib txuas thiab kev sib cuam tshuam ntawm cov protein. Los ntawm kev sau cov protein tshwj xeeb nrog FITC, cov kws tshawb nrhiav tuaj yeem tshuaj xyuas lawv qhov kev sib cuam tshuam siv cov txheej txheem xws li fluorescence resonance energy transfer (FRET) lossis fluorescence polarization.

Kev taug qab Biomolecule: FITC siv tau los taug qab thiab taug qab kev txav mus los ntawm biomolecules hauv cov hlwb lossis cov nqaij uas muaj sia nyob. Los ntawm kev sau cov molecules uas koj nyiam nrog FITC, cov kws tshawb fawb tuaj yeem kawm lawv qhov chaw nyob, kev hloov pauv, thiab cov qauv kev thauj mus los hauv lub sijhawm tiag tiag siv lub tshuab fluorescence microscopy.

Cov ntawv thov kuaj mob: Cov probes lossis cov tshuaj tiv thaiv kab mob uas muaj FITC siv rau hauv kev kuaj mob rau ntau yam kev siv, suav nrog kev kuaj pom cov antigens tshwj xeeb, cov neeg kis kab mob, lossis cov cim qhia hauv cov qauv ntawm tus neeg mob. Cov probes fluorescent no muab kev kuaj pom rhiab heev thiab tshwj xeeb, ua rau kev kuaj mob sai thiab raug.