Phenylgalactoside, tseem hu ua p-nitrophenyl β-D-galactopyranoside (pNPG), yog ib qho hluavtaws substrate nquag siv hauv biochemical thiab molecular biology thwmsim.Nws yog feem ntau siv los kuaj thiab ntsuas qhov ua haujlwm ntawm enzyme β-galactosidase.
Thaum phenylgalactoside yog hydrolyzed los ntawm β-galactosidase, nws tso tawm p-nitrophenol, uas yog xim daj xim.Kev ywj pheej ntawm p-nitrophenol tuaj yeem ntsuas qhov ntau ntawm kev siv spectrophotometer, vim tias qhov nqus ntawm p-nitrophenol tuaj yeem kuaj pom ntawm lub wavelength ntawm 405 nm.