Disodium 4-nitrophenylphosphate CAS: 4264-83-9

Qhov cuam tshuam tseem ceeb thiab kev siv ntawm disodium 4-nitrophenylphosphate yog nws siv ua lub substrate chromogenic rau alkaline phosphatase enzyme assays. Thaum cov tshuaj no raug incubated nrog alkaline phosphatase, cov enzyme txiav cov phosphate pawg ntawm disodium 4-nitrophenylphosphate, ua rau muaj 4-nitrophenol.

4-nitrophenol uas tsim tawm yog ib qho xim daj uas tuaj yeem pom tau los ntawm spectrophotometrically ntawm 405 nm. Qhov kev hloov xim no yog siv los ntsuas qhov kev ua haujlwm ntawm alkaline phosphatase hauv cov qauv, xws li cov kua dej hauv lub cev lossis cov cell lysates.

Kev siv disodium 4-nitrophenylphosphate hauv kev ntsuam xyuas enzyme muab ib txoj hauv kev yooj yim rau kev ntsuas alkaline phosphatase kev ua haujlwm. Cov enzyme no koom nrog ntau yam txheej txheem biological thiab feem ntau yog siv ua lub cim rau kev sib txawv ntawm cov ntaub so ntswg, kev ua haujlwm ntawm daim siab, thiab kev hloov pauv pob txha.

Tsis tas li ntawd xwb, disodium 4-nitrophenylphosphate tuaj yeem siv ua lub substrate rau lwm cov phosphatases, xws li acid phosphatase lossis protein phosphatases, nyob ntawm qhov kev teeb tsa sim thiab lub hom phiaj tshawb fawb.