DEPC CAS: 1609-47-8 Tus nqi tsim khoom

Diethyl pyrocarbonate (DEPC) yog ib yam tshuaj lom neeg uas feem ntau siv hauv cov chaw kuaj mob molecular biology. Nws yog ib yam kua ntshiab thiab tsis muaj xim uas muaj kev cuam tshuam ntau rau cov tshuaj nucleophilic xws li amines thiab thiols. DEPC feem ntau yog siv los ua kom RNases (ribonucleases), cov enzymes uas tuaj yeem ua rau RNA puas tsuaj, los ntawm kev hloov kho lawv cov chaw ua haujlwm. Nws ua tiav qhov no los ntawm kev cuam tshuam nrog histidine residues hauv cov enzyme, uas yog qhov tseem ceeb rau nws cov haujlwm. Qhov no ua rau DEPC yog ib qho cuab yeej muaj txiaj ntsig hauv kev sim RNA thiab ua kom muaj kev tiv thaiv ntawm RNA kev ncaj ncees. Ntxiv nrog rau nws cov khoom RNase inactivating, DEPC kuj tseem tuaj yeem hloov kho lwm yam biomolecules, xws li cov protein thiab DNA, vim nws cov tshuaj tiv thaiv nrog cov pab pawg nucleophilic. Yuav tsum ceev faj thaum tuav DEPC, vim nws yog tshuaj lom thiab muaj peev xwm mutagenic.

Txiav txim los yog Thov Kev Hais Nqe

Cov Lus Qhia Txog Khoom

Cov Cim Npe Khoom

Daim Ntawv Thov thiab Cov Nyhuv

Kev Ua Kom RNases Tsis Ua Haujlwm: Lub hom phiaj tseem ceeb ntawm DEPC yog kom ua kom RNases tsis ua haujlwm, uas yog cov enzymes uas tuaj yeem ua rau RNA puas tsuaj. DEPC teb nrog histidine residues hauv RNases, hloov kho lawv cov chaw ua haujlwm thiab ua rau lawv tsis ua haujlwm. Qhov no ua kom ntseeg tau tias kev khaws cia ntawm RNA kev ncaj ncees thaum lub sijhawm sim xws li RNA cais tawm, rov qab sau ntawv, thiab RNA sequencing.

Kev Khaws Cov Qauv RNA: Kev kho mob DEPC feem ntau siv rau cov khoom siv hauv chaw kuaj mob, xws li cov raj microcentrifuge, cov lus qhia pipette, thiab dej siv rau hauv kev sim RNA. Qhov no pab tiv thaiv txhua qhov seem RNases uas yuav muaj thiab tiv thaiv RNA degradation thaum lub sijhawm coj mus kuaj thiab khaws cia.

Kev npaj cov tshuaj reagent uas tsis muaj RNAse: DEPC siv tau los kho cov tshuaj lossis cov tshuaj reagents uas yuav tsum tsis muaj RNAse, xws li cov buffers, Tris-EDTA (TE) cov tshuaj, lossis dej siv rau hauv kev sim molecular biology. Cov tshuaj kho no ces siv tau kom muaj kev nyab xeeb los txo qhov kev puas tsuaj ntawm RNA.

Kev hloov kho DNA: DEPC kuj tseem tuaj yeem cuam tshuam nrog cov pab pawg amino hauv cov DNA molecules, ua rau muaj kev hloov kho carbethoxylated ntawm DNA bases. Qhov no tuaj yeem siv rau hauv cov ntawv thov tshwj xeeb, xws li DNA sequencing reactions lossis hloov kho DNA rau kev tshawb fawb crosslinking.

Khoom Ntim:

Cov Lus Qhia Ntxiv:

Kev sau ua ke	C6H10O5
Kev Ntsuas	99%
Qhov tsos	kua tsis muaj xim
CAS Nr.	1609-47-8
Ntim khoom	Me me thiab ntau
Lub Neej Txee	2 xyoos
Kev Khaws Cia	Khaws cia rau qhov chaw txias thiab qhuav
Daim Ntawv Pov Thawj	ISO.