BCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine ntsev CAS: 6578-06-9

Western blotting: BCIP yog siv los nrhiav thiab pom cov protein uas tau hloov mus rau ntawm daim nyias nyias tom qab gel electrophoresis. Cov tshuaj tiv thaiv kab mob theem ob uas muaj alkaline phosphatase txuas nrog yog siv los khi rau cov tshuaj tiv thaiv kab mob thawj zaug, thiab tom qab ntawd nrog BCIP ua rau muaj cov dej xiav ntawm qhov chaw ntawm cov protein bands.

Kev Tiv Thaiv Kab Mob: BCIP siv rau hauv cov txheej txheem immunohistochemical staining los ntes qhov muaj cov protein tshwj xeeb hauv cov nqaij. Cov tshuaj tiv thaiv kab mob theem ob uas sib txuas nrog Alkaline phosphatase yog siv los khi rau cov tshuaj tiv thaiv kab mob thawj zaug, thiab kev kho mob BCIP tsim cov xim xiav thaum muaj cov enzyme, qhia txog qhov chaw nyob ntawm cov protein hauv cov qauv nqaij.

Kev sib xyaw ua ke hauv qhov chaw: BCIP kuj tseem siv rau kev ntes cov kab ke nucleic acid hauv cov nqaij lossis cov hlwb ruaj khov. Cov tshuaj ntsuam sib xyaw ua ke tau muab cim nrog alkaline phosphatase, thiab thaum ntxiv BCIP, qhov muaj nyob ntawm lub hom phiaj mRNA lossis DNA yog qhia los ntawm kev txhim kho ntawm xim xiav.