4-Nitrophenyl-2-acetamido-2-deoxy-β-D-glucopyranoside CAS: 3459-18-5

Enzyme Substrate: pNAG yog dav siv los ua ib qho substrate rau ntau yam enzymes koom nrog hauv hydrolysis ntawm β-D-glucoside bonds.Thaum cov enzymes no tshem tawm pNAG molecule, nws tso p-nitrophenol.Qhov no tso cai rau cov kws tshawb fawb los ntsuas thiab ntsuas qhov ua haujlwm enzymatic.

Enzyme Activity Assays: Lub hydrolysis ntawm pNAG los ntawm cov enzymes tshwj xeeb tuaj yeem kuaj pom thiab ntsuas spectrophotometrically.Qhov no ua rau pNAG haum rau kev soj ntsuam kev ua enzyme, qhov twg tus nqi ntawm p-nitrophenol generated yog ncaj qha proportional rau lub enzymatic kev ua si.

High-Throughput Screening: pNAG feem ntau yog siv rau hauv kev tshuaj ntsuam xyuas siab los txheeb xyuas thiab ua tus cwj pwm enzyme inhibitors lossis activators.Los ntawm kev ntsuam xyuas cov txiaj ntsig ntawm cov tshuaj sib txawv ntawm cov kev ua haujlwm enzymatic, cov kws tshawb fawb tuaj yeem txheeb xyuas cov neeg siv tshuaj muaj peev xwm lossis cov kev hloov pauv ntawm cov enzymes.

Gene Expression Studies: pNAG kuj tseem siv rau hauv kev tshawb fawb molecular biology los kawm txog noob caj noob ces thiab kev cai.Los ntawm kev ntsuas cov kev ua ntawm cov enzymes tshwj xeeb uas siv pNAG ua lub substrate, cov kws tshawb fawb tuaj yeem tshawb xyuas cov txiaj ntsig ntawm cov noob qhia txog kev ua haujlwm enzyme thiab kev ua haujlwm.