4-Amino-3-hydrazino-1,2,4-triazol-5-thiol CAS: 28836-03-5

Qhov cuam tshuam ntawm 3,3'-Diaminobenzidine (DAB) yog los tsim xim av thaum nws cuam tshuam nrog cov enzymes peroxidase. Qhov kev hloov xim no tso cai rau kev pom ntawm cov antigens lossis biomolecules uas txaus siab rau immunohistochemistry (IHC) thiab immunocytochemistry (ICC) staining assays. DAB feem ntau siv rau hauv kev tshawb fawb biomedical thiab kev siv tshuaj ntsuam xyuas kom pom thiab nrhiav cov antigens lossis cov protein tshwj xeeb hauv cov qauv tshuaj lom neeg. Los ntawm kev ntxiv DAB ua lub substrate thiab tom qab ntawd cuam tshuam nrog cov enzymes peroxidase, cov xim av precipitate yog tsim ntawm qhov chaw ntawm cov antigens. Daim ntawv thov ntawm DAB staining yog qhov tshwj xeeb tshaj yog pab tau hauv kev kawm ntau yam kab mob thiab tej yam mob. Nws ua rau cov kws tshawb fawb thiab cov kws kho mob pom thiab txheeb xyuas qhov kev faib tawm, kev muaj ntau, thiab qhov chaw ntawm cov biomolecules tshwj xeeb hauv cov ntaub so ntswg lossis cov hlwb. Cov ntaub ntawv no pab kom nkag siab txog cov txheej txheem ntawm tus kab mob, kawm txog cov txheej txheem ntawm lub cev, thiab txheeb xyuas cov hom phiaj kuaj mob lossis kho mob. Ntxiv mus, kev siv DAB staining tuaj yeem siv rau hauv ntau txoj kev siv molecular los tshawb nrhiav kev sib cuam tshuam ntawm cov protein-protein, txheeb xyuas cov qauv kev qhia tawm ntawm cov protein, txiav txim siab seb puas muaj cov cim tshwj xeeb, thiab ntsuas qhov ua tau zoo ntawm kev kho mob. Vim nws cov staining txhim khu kev qha thiab muaj zog, nrog rau nws qhov sib xws nrog ntau yam kev kuaj pom thiab cov txheej txheem microscopy, DAB tau dhau los ua cov khoom siv chromogenic siv dav hauv immunohistochemistry thiab immunocytochemistry assays.