3,3′,5,5′-Tetramethylbenzidine sulfate CAS: 54827-18-8

ELISA: Nws yog siv dav ua lub substrate rau horseradish peroxidase (HRP) hauv ELISA assays. Thaum muaj lub hom phiaj molecule, xws li antigen lossis antibody, lub enzyme HRP catalyzes qhov oxidation ntawm 3,3', 5,5'-Tetramethylbenzidine sulfate, ua rau muaj cov khoom xiav. Qhov muaj zog ntawm cov xim yog ncaj qha proportional rau qhov concentration ntawm lub hom phiaj molecule, tso cai rau kev tshuaj xyuas ntau.

Kev ntsuam xyuas kev ua haujlwm ntawm enzyme: 3,3',5,5'-Tetramethylbenzidine sulfate siv tau los ntsuas kev ua haujlwm ntawm enzyme. Los ntawm kev sib xyaw cov qauv enzyme nrog lub substrate, qhov kev ua haujlwm ntawm enzymatic tuaj yeem saib xyuas los ntawm kev hloov pauv xim. Kev ua haujlwm ntawm enzyme siab dua ua rau muaj xim xiav ntau dua, ua rau muaj kev txiav txim siab ntawm enzyme kinetics thiab kev tshawb fawb txog kev txwv tsis pub ua.

Immunohistochemistry: Nws tuaj yeem siv rau cov txheej txheem staining hauv immunohistochemistry (IHC). Nws yog siv ua chromogen rau horseradish peroxidase-labeled thawj lossis theem ob antibodies, ua rau muaj kev tsim ntawm cov xim xiav pom tseeb ntawm qhov chaw ntawm antigen-antibody sib cuam tshuam.

Kev tshawb fawb txog biochemical: 3,3',5,5'-Tetramethylbenzidine sulfate pom muaj kev siv rau hauv ntau yam kev tshawb fawb txog biochemical, tshwj xeeb tshaj yog hauv kev nrhiav thiab ntsuas cov haujlwm ntawm enzyme, kev sib cuam tshuam ntawm cov protein-protein, thiab kev sib cuam tshuam ntawm cov protein-DNA.