3,3,5,5-Tetramethylbenzidine dihydrochloride hydrate CAS: 207738-08-7

3,3',5,5'-Tetramethylbenzidine dihydrochloride (TMB) yog ib qho khoom siv dav dav hauv kev ntsuam xyuas enzyme-linked immunosorbent assays (ELISAs) thiab lwm yam kev ntsuam xyuas biochemical. Thaum TMB raug oxidized los ntawm enzyme horseradish peroxidase (HRP) nyob rau hauv qhov muaj hydrogen peroxide, nws tsim cov xim xiav. Qhov kev hloov xim no yog siv los ntes qhov muaj cov tshuaj tiv thaiv HRP lossis lwm cov protein, feem ntau qhia txog qhov muaj cov antigen tshwj xeeb hauv cov qauv. Cov xim xiav uas tsim los ntawm cov tshuaj feem ntau yog ntsuas siv lub spectrophotometer, uas tso cai rau kev ntsuas qhov muaj zog ntawm kev hloov xim thiab muab ib txoj hauv kev los sib piv qhov muaj zog ntawm cov xim nrog rau qhov ntau ntawm cov molecule uas muaj nyob hauv cov qauv. TMB muaj ntau daim ntawv thov hauv kev ntsuam xyuas kev kuaj mob, kev tshawb fawb, thiab kev tsim tshuaj, tshwj xeeb tshaj yog hauv kev ntes cov tshuaj tiv thaiv, cov tshuaj hormones, cytokines, thiab lwm yam biomolecules. Nws qhov kev rhiab heev, kev ruaj khov, thiab kev yooj yim ntawm kev siv ua rau nws yog ib qho cuab yeej muaj txiaj ntsig hauv ntau qhov chaw kuaj mob rau kev ntes thiab ntsuas cov molecules tshwj xeeb.