3-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE CAS TSIS TAU: 3150-25-2

Kev kuaj xyuas ntawm beta-galactosidase kev ua haujlwm: ONPG feem ntau siv los txiav txim siab txog qhov muaj thiab kev ua ntawm beta-galactosidase nyob rau hauv ntau yam kab mob lom, xws li kab mob kab mob los yog cell lysates.Kev tsim cov o-nitrophenol, uas muaj xim daj, tuaj yeem ntsuas tau yooj yim siv lub spectrophotometer.

Cov kev tshawb fawb txog noob caj noob ces: ONPG feem ntau yog siv hauv kev tshawb fawb molecular biology los kawm txog cov noob caj noob ces.Los ntawm kev sib txuas cov neeg txhawb nqa ntawm cov noob ntawm kev txaus siab nrog cov noob encoding beta-galactosidase, cov kws tshawb fawb tuaj yeem ntsuas qhov kev ua haujlwm ntawm tus neeg txhawb nqa no los ntawm kev ntxiv ONPG thiab ntsuas qhov ua rau o-nitrophenol ntau lawm.Txoj kev no, hu ua beta-galactosidase reporter assay, muab cov ntaub ntawv hais txog kev ua haujlwm ntawm cov noob caj noob ces.

Kev txheeb xyuas cov kab mob: Qee cov kab mob tsim beta-galactosidase, thaum lwm tus tsis ua.ONPG tuaj yeem siv ua ke nrog lwm yam kev sim tshuaj biochemical los txheeb xyuas cov kab mob raws li lawv lub peev xwm los hydrolyze ONPG.Txoj kev no feem ntau yog siv rau hauv kev kuaj mob kuaj mob thiab kuaj microbiology.

Kev tshuaj ntsuam rau enzyme inhibitors lossis activators: ONPG tuaj yeem siv los tshuaj xyuas cov tshuaj sib xyaw uas hloov pauv cov haujlwm ntawm beta-galactosidase.Los ntawm kev ntsuas cov kev ua enzyme nyob rau hauv muaj cov sib txuas sib txawv, cov kws tshawb fawb tuaj yeem txheeb xyuas cov inhibitors lossis activators uas tuaj yeem tshawb xyuas ntxiv rau lawv cov peev xwm kho tau.