3-(cyclohexylamino)-2-hydroxy-1-propanesuhicic acid CAS: 73463-39-5

CAPSO (3-(cyclohexylamino)-2-hydroxypropanesulfonic acid) yog ib qho zwitterionic buffer uas feem ntau siv hauv biochemistry thiab molecular biology. Nws paub txog nws lub peev xwm buffering siab dhau ntawm ntau yam pH thiab suav tias yog ib qho kev hloov pauv zoo rau lwm cov buffers xws li MOPS thiab MES.

Qhov cuam tshuam tseem ceeb ntawm CAPSO yog nws lub peev xwm los tswj kom muaj pH ib puag ncig ruaj khov hauv kev sim tshuaj lom neeg. Nws ua haujlwm los ntawm kev pub lossis txais cov protons kom txo qis kev hloov pauv hauv pH los ntawm cov kua qaub lossis cov hauv paus ntxiv. Nws tus nqi pKa yog nyob ib puag ncig 9.8, uas ua rau nws yog ib qho buffer zoo rau kev sim hauv pH ntau ntawm 8.2-9.6.

CAPSO feem ntau siv rau hauv cov ntawv thov xws li kev ntxuav cov protein, kev ntsuam xyuas enzyme, thiab electrophoresis. Nws qhov kev ruaj khov thiab kev cuam tshuam tsawg kawg nkaus nrog cov tshuaj tiv thaiv kab mob ua rau nws muaj txiaj ntsig zoo rau kev tswj hwm qhov zoo tshaj plaws rau cov tshuaj tiv thaiv biochemical. Tsis tas li ntawd, CAPSO feem ntau siv rau kev kawm txog cov yam ntxwv ntawm cov protein yooj yim, kev sib sau ua ke ntawm cov protein, thiab kev ruaj khov.