2-NITROPHENYL-BETA-D-GLUCOPYRANOSIDE CAS: 2816-24-2

Cov khoom siv enzyme: ONPG feem ntau siv ua cov khoom siv rau beta-galactosidase, ib qho enzyme uas hydrolyzes ONPG los tsim cov tshuaj daj (o-nitrophenol) uas tuaj yeem pom tau yooj yim los ntawm spectrophotometrically. Cov tshuaj tiv thaiv enzymatic no siv tau los ntsuas qhov kev ua haujlwm ntawm beta-galactosidase, ua rau ONPG yog ib qho cuab yeej muaj txiaj ntsig zoo hauv kev tshawb fawb enzymology.

Kev ntsuam xyuas molecular biology: ONPG yog siv ua lub substrate hauv ntau yam kev ntsuam xyuas molecular biology, tshwj xeeb tshaj yog hauv beta-galactosidase reporter gene assays. Hauv cov kev ntsuam xyuas no, ib qho ONPG-based substrate yog siv los ntsuas qhov kev ua haujlwm ntawm tus reporter gene, uas feem ntau yog tswj hwm los ntawm ib qho kev txhawb nqa tshwj xeeb. Qhov kev ua haujlwm beta-galactosidase, qhia los ntawm kev hloov xim tsim tawm thaum ONPG hydrolysis, muab cov ntaub ntawv tseem ceeb txog kev ua haujlwm ntawm tus txhawb nqa.

Kev tshuaj xyuas kev qhia tawm ntawm cov noob caj noob ces: ONPG kuj tseem siv rau hauv kev tshuaj xyuas kev qhia tawm ntawm cov noob caj noob ces. Los ntawm kev txuas cov kab ke ntawm kev txhawb nqa rau cov noob caj noob ces beta-galactosidase, cov kws tshawb fawb tuaj yeem ntsuas qhov kev ua haujlwm ntawm beta-galactosidase siv ONPG ua lub substrate. Qib ntawm kev ua haujlwm ntawm beta-galactosidase yog qhov cuam tshuam ntawm lub zog thiab kev ua haujlwm ntawm tus txhawb nqa, uas tso cai rau kev ntsuam xyuas cov qib kev qhia tawm ntawm cov noob caj noob ces.

Cov ntawv thov kuaj mob: ONPG tuaj yeem siv rau hauv kev siv kuaj mob, tshwj xeeb tshaj yog hauv kev kuaj pom cov kab mob pathogenic. Ntau yam kab mob, xws li Escherichia coli thiab qee hom Shigella thiab Salmonella, tsim cov beta-galactosidase uas tuaj yeem txiav ONPG. Qhov kev hloov pauv hydrolysis no ua rau pom kev hloov xim, uas tuaj yeem siv los txiav txim siab qhov muaj cov kab mob no hauv cov qauv kuaj mob.