PIPES CAS: 5625-37-6 Chaw tsim tshuaj paus Nqe

PIPES (piperazine-1,4-bisethanesulfonic acid) yog ib qho zwitterionic buffering compound feem ntau siv hauv kev tshawb fawb lom thiab biochemical.Nws muaj ntau yam tseem ceeb thiab kev siv, suav nrog:

pH buffering tus neeg sawv cev: PIPES yog qhov tsis zoo uas pab tswj cov pH ruaj khov hauv ntau yam kev sim tshuaj lom neeg.Nws yog feem ntau siv rau hauv kab lis kev cai xov xwm ntawm tes, kev tshuaj ntsuam enzyme, thiab daim ntawv thov molecular biology.

Muaj peev xwm buffering siab: PIPES muaj peev xwm buffering zoo nyob rau hauv thaj tsam pH ntawm 6.1 txog 7.5, ua rau nws tsim nyog rau kev tswj xyuas pH ruaj khov hauv ntau hom kab mob lom.

Kev sib cuam tshuam tsawg kawg nkaus nrog biomolecules: PIPES paub txog nws qhov cuam tshuam tsawg ntawm cov txheej txheem biochemical thiab kev khi me me rau cov proteins thiab cov enzymes, ua rau nws zoo tagnrho rau kev tswj xyuas kev ncaj ncees thiab kev ua haujlwm ntawm biomolecules.

Tsim nyog rau qhov ntsuas kub raws li qhov ntsuas kub: PIPES tuaj yeem khaws nws cov khoom buffering nyob rau hauv ntau qhov kub thiab txias, suav nrog ob qho tib si physiological thiab siab kub.Qhov no ua rau nws tsim nyog rau cov kev sim uas xav tau kev ruaj ntseg thiab qhov tseeb hauv qhov kub thiab txias.

Electrophoresis daim ntaub ntawv: PIPES feem ntau yog siv los ua ib qho tsis nyob rau hauv gel electrophoresis cov tswv yim, xws li RNA los yog DNA agarose gel electrophoresis, vim nws tsis tshua muaj UV absorbance thiab siab conductivity zog.

Kev tsim tshuaj: PIPES kuj tseem ua haujlwm hauv kev tsim tshuaj raws li tus neeg sawv cev buffering, muab kev ruaj ntseg thiab tswj xyuas qhov pH zoo rau kev siv tshuaj.