Piperazine-1,4-bis (2-ethanesulfonic acid) disodium ntsev CAS: 76836-02-7

Cov teebmeem:

Buffering zog: PIPES tuaj yeem siv los tswj cov qib pH tas li nyob rau hauv ib qho kev tshwj xeeb, vim nws muaj txiaj ntsig zoo ntawm buffering hauv physiological pH ntau ntawm 6.1-7.5.Qhov no ua rau nws muaj txiaj ntsig hauv ntau yam kev sim tshuaj lom neeg uas tswj pH tseem ceeb.

Stability: PIPES yog ruaj khov nyob rau hauv ntau qhov kub thiab txias, ua rau nws haum rau siv nyob rau hauv kev sim ua nyob rau hauv ntau yam kev mob.

Daim ntawv thov:

Kab lis kev cai ntawm tes: PIPES tuaj yeem siv los ua qhov tsis nyob hauv cov txheej txheem kab lis kev cai ntawm tes, xws li kev tswj hwm pH ntawm cov xov xwm lossis cov buffers siv rau kev loj hlob ntawm tes thiab kev saib xyuas.

Protein thiab enzyme kev tshawb fawb: PIPES feem ntau yog siv rau hauv cov protein thiab enzyme kev tshawb fawb kom muaj pH ruaj khov thaum muaj kev cuam tshuam ntau yam, tshwj xeeb tshaj yog cov uas muaj cov enzymes rhiab heev lossis cov proteins uas yuav cuam tshuam los ntawm kev hloov pH.

Electrophoresis: PIPES tuaj yeem siv los ua qhov tsis nyob hauv daim ntawv thov gel electrophoresis, pab tswj cov pH zoo rau DNA lossis protein sib cais.

Molecular biology cov txheej txheem: PIPES tuaj yeem siv los ua qhov tsis nyob hauv ntau cov txheej txheem molecular biology, suav nrog DNA / RNA extraction, PCR, thiab DNA sequencing, kom ntseeg tau qhov tseeb los ntawm kev tswj cov pH ruaj khov.