Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside CAS: 24404-53-3

Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside feem ntau yog siv los ua substrate rau enzyme β-galactosidase.Thaum β-galactosidase ua rau nws, cov compound yog hydrolyzed, ua rau zus tau tej cov p-nitrophenol los yog o-nitrophenol, uas muaj xim daj uas tuaj yeem tshawb pom tau yooj yim.Qhov no ua rau nws muaj txiaj ntsig zoo li substrate rau kev soj ntsuam uas ntsuas β-galactosidase kev ua haujlwm.

Ib daim ntawv thov ntawm Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside yog nyob rau hauv reporter gene assays.Hauv cov kev ntsuam xyuas no, cov noob ntawm kev txaus siab yog ua ke rau cov neeg sau xov xwm gene, xws li cov noob beta-galactosidase.Cov kev ua ntawm cov neeg sau xov xwm gene yog saib xyuas los ntawm kev ntsuas β-galactosidase kev ua haujlwm siv Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside ua cov substrate.Qhov no tso cai rau cov kws tshawb fawb los kawm txog gene qhia thiab kev tswj hwm.

Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside kuj tuaj yeem siv los tshuaj xyuas cov inhibitors lossis activators ntawm β-galactosidase.Los ntawm kev sim cov tshuaj sib txawv lossis cov neeg sib tw tshuaj, cov kws tshawb fawb tuaj yeem txheeb xyuas cov molecules uas hloov cov haujlwm ntawm cov enzyme no, uas tuaj yeem muaj kev siv tshuaj kho mob hauv qee yam kab mob lossis kev mob.

Tsis tas li ntawd, qhov sib xyaw no tuaj yeem siv rau hauv kev ua kom huv ntawm cov protein.β-galactosidase feem ntau yog fused rau cov proteins uas txaus siab los pab txhawb lawv qhov kev kuaj pom thiab ua kom huv.Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside tuaj yeem siv los ua lub substrate los tshuaj xyuas thiab ntxuav cov fusion proteins los ntawm kev kuaj xyuas cov haujlwm β-galactosidase.