IPTG CAS: 367-93-1 Chaw tsim tshuaj paus Nqe
Isopropyl β-D-1-thiogalactopyranoside (IPTG) yog ib qho hluavtaws analogue ntawm lactose uas feem ntau siv rau hauv kev tshawb fawb molecular biology thiab kev siv biotechnology.IPTG feem ntau yog siv los txhawb kev qhia cov noob hauv cov kab mob hauv cov kab mob, qhov chaw uas nws ua haujlwm los ua cov tshuaj molecular los pib qhov kev hloov pauv ntawm cov hom phiaj.
Thaum ntxiv rau qhov kev loj hlob nruab nrab, IPTG yog coj los ntawm cov kab mob thiab tuaj yeem khi rau cov protein lac repressor, tiv thaiv nws los ntawm kev thaiv kev ua haujlwm ntawm lac operon.Lub lac operon yog ib pawg ntawm cov noob koom nrog hauv cov metabolism hauv lactose, thiab thaum tshem tawm cov protein repressor, cov noob tau nthuav tawm.
IPTG feem ntau yog siv ua ke nrog lacUV5 mutant promoter, uas yog ib qho kev sib koom ua ke ntawm lac txhawb nqa.Los ntawm kev sib koom ua ke IPTG induction nrog qhov kev txhawb nqa mutant, cov kws tshawb fawb tuaj yeem ua tiav qib siab ntawm cov noob qhia.Qhov no tso cai rau kev tsim cov protein ntau ntau rau kev ua kom huv lossis lwm yam kev siv qis.
Ntxiv nrog rau kev qhia cov noob, IPTG kuj nquag siv hauv kev tshuaj ntsuam xiav / dawb.Hauv cov txheej txheem no, lacZ gene feem ntau yog fused rau cov noob ntawm kev txaus siab, thiab cov kab mob uas ua tiav cov fusion gene yuav tsim ib qho active β-galactosidase enzyme.Thaum IPTG ntxiv nrog rau cov chromogenic substrate xws li X-gal, cov kab mob uas qhia cov fusion gene tig xiav vim yog cov haujlwm ntawm β-galactosidase.Qhov no tso cai rau kev txheeb xyuas thiab xaiv cov kab mob sib xyaw ua ke uas tau ua tiav kev sib xyaw ntawm cov noob paj.
Induction ntawm cov noob qhia: IPTG feem ntau yog siv los ntxias kev qhia ntawm cov hom phiaj hauv cov kab mob.Nws ua raws li ntuj tsim cov lactose thiab khi rau cov lac repressor protein, tiv thaiv nws los ntawm kev thaiv cov lac operon.Qhov no tso cai rau transcription thiab qhia txog cov noob xav tau.
Protein nthuav qhia thiab purification: IPTG induction feem ntau yog siv los tsim ntau cov proteins recombinant rau ntau lub hom phiaj, xws li kev tshawb fawb txog biochemical, kev kho mob, lossis kev soj ntsuam cov qauv.Los ntawm kev siv cov vectors tsim nyog thiab IPTG induction, cov kws tshawb fawb tuaj yeem ua tiav qib siab ntawm cov hom phiaj tsim cov protein ntau hauv cov kab mob.
Kev tshuaj ntsuam xiav / dawb: IPTG nquag siv ua ke nrog lacZ gene thiab chromogenic substrate, xws li X-gal, rau kev tshuaj ntsuam xiav / dawb.Lub lacZ gene feem ntau fused rau cov noob ntawm kev txaus siab, thiab cov kab mob uas ua tiav cov fusion gene yuav tsim ib qho active β-galactosidase enzyme.Thaum IPTG thiab cov chromogenic substrate raug ntxiv, cov kab mob sib xyaw ua ke qhia cov fusion gene tig xiav, tso cai rau kev txheeb xyuas yooj yim thiab xaiv.
Kawm txog kev tswj cov noob: IPTG induction feem ntau siv rau hauv kev tshawb fawb los kawm txog kev tswj cov noob thiab cov neeg ua haujlwm, tshwj xeeb tshaj yog cov lac operon.Los ntawm kev tswj hwm qhov ntau ntawm IPTG thiab saib xyuas cov lus qhia ntawm lac operon Cheebtsam, cov kws tshawb fawb tuaj yeem tshawb xyuas cov txheej txheem ntawm kev tswj cov noob thiab lub luag haujlwm ntawm ntau yam lossis kev hloov pauv.
Gene expression systems: IPTG yog ib qho tseem ceeb hauv ntau lub tshuab qhia cov noob, xws li T7 cov txheej txheem txhawb nqa.Hauv cov kab ke no, lac txhawb nqa feem ntau yog siv los tsav cov kev qhia ntawm T7 RNA polymerase, uas, dhau los, sau cov noob caj noob ces raws li kev tswj hwm ntawm T7 cov txheej txheem txhawb nqa.IPTG yog siv los ntxias cov kev qhia ntawm T7 RNA polymerase, ua rau kev ua kom lub hom phiaj gene qhia.
| Kev sib xyaw | C9H18O5S |
| Assay | 99% |
| Qhov tshwm sim | Dawb hmoov |
| CAS Nr. | 367-93-1 : kuv |
| Ntim | Me me thiab bulk |
| Txee lub neej | 2 xyoo |
| Cia | Khaws rau hauv qhov chaw txias thiab qhuav |
| Daim ntawv pov thawj | ISO. |
